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Analysis of Flavonoids in Lotus (Nelumbo nucifera) Leaves and Their Antioxidant Activity Using Macroporous Resin Chromatography Coupled with LC-MS/MS and Antioxidant Biochemical Assays
文献类型：期刊论文
| 作者 | Wu, Wei1; Zhu, Ming-Zhi2; Guo, Ming-Quan2; Yang, Ping-Fang2; Jiao, Li-Li1 |
| 刊名 | MOLECULES
 |
| 出版日期 | 2015-06-01 |
| 卷号 | 20期号:6页码:10553-10565 |
| 关键词 | lotus leaves antioxidant activity macroporous resin chromatography mass spectrometry |
| ISSN号 | 1420-3049 |
| DOI | 10.3390/molecules200610553 |
| 英文摘要 | Lotus (Nelumbo nucifera) leaves, a traditional Chinese medicinal herb, are rich in flavonoids. In an effort to thoroughly analyze their flavonoid components, macroporous resin chromatography coupled with HPLC-MS/MS was employed to simultaneously enrich and identify flavonoids from lotus leaves. Flavonoids extracted from lotus leaves were selectively enriched in the macroporous resin column, eluted subsequently as fraction II, and successively subjected to analysis with the HPLC-MS/MS and bioactivity assays. Altogether, fourteen flavonoids were identified, four of which were identified from lotus leaves for the first time, including quercetin 3-O-rhamnopyranosyl-(12)-glucopyranoside, quercetin 3-O-arabinoside, diosmetin 7-O-hexose, and isorhamnetin 3-O-arabino- pyranosyl-(12)-glucopyranoside. Further bioactivity assays revealed that these flavonoids from lotus leaves possess strong antioxidant activity, and demonstrate very good potential to be explored as food supplements or even pharmaceutical products to improve human health. |
| 资助项目 | "Hundred Talents Program" from Chinese Academy of Sciences[29Y429291a0129] ; Knowledge Innovation Project of Chinese Academy of Sciences[Y455421Z02] ; Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture of Wuhan Botanical Garden, Chinese Academy of Sciences |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000357992700065 |
| 出版者 | MDPI AG |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/252]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Guo, Ming-Quan |
| 作者单位 | 1.Changchun Univ Chinese Tradit Med, Changchun 130000, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Wei,Zhu, Ming-Zhi,Guo, Ming-Quan,et al. Analysis of Flavonoids in Lotus (Nelumbo nucifera) Leaves and Their Antioxidant Activity Using Macroporous Resin Chromatography Coupled with LC-MS/MS and Antioxidant Biochemical Assays[J]. MOLECULES,2015,20(6):10553-10565. |
| APA | Wu, Wei,Zhu, Ming-Zhi,Guo, Ming-Quan,Yang, Ping-Fang,&Jiao, Li-Li.(2015).Analysis of Flavonoids in Lotus (Nelumbo nucifera) Leaves and Their Antioxidant Activity Using Macroporous Resin Chromatography Coupled with LC-MS/MS and Antioxidant Biochemical Assays.MOLECULES,20(6),10553-10565. |
| MLA | Wu, Wei,et al."Analysis of Flavonoids in Lotus (Nelumbo nucifera) Leaves and Their Antioxidant Activity Using Macroporous Resin Chromatography Coupled with LC-MS/MS and Antioxidant Biochemical Assays".MOLECULES 20.6(2015):10553-10565. |
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来源：武汉植物园
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