An insertion-deletion at a pentatricopeptide repeat locus linked to fertility transition to cytoplasmic male sterility in radish (Raphanus sativus L.)
文献类型：期刊论文
| 作者 | Wang, Ting3; Zhou, Yuan3; Gao, Lei3; Mei, Shi Yong1; Wang, Chuan De2,3; Wang, Zhi Wei3 |
| 刊名 | MOLECULAR BREEDING
 |
| 出版日期 | 2015-04-01 |
| 卷号 | 35期号:4页码:5 |
| 关键词 | Cytoplasmic male sterility Fertility restoration Insertion-deletion Pentatricopeptide repeat |
| ISSN号 | 1380-3743 |
| DOI | 10.1007/s11032-015-0301-x |
| 英文摘要 | The cytoplasmic male sterility (CMS)/nucleus-controlled fertility restoration (Rf) system has been widely applied in hybrid seed production and is an ideal model for dissecting the interaction between the nucleus and the mitochondrion. In this study, we identified an insertion-deletion (InDel) at a male fertility restoration locus (RsRf3) for CMS in radish (Raphanus sativus L.). A pentatricopeptide repeat (PPR)-encoding allele RsRf3-4 was found and encodes a predicted protein 579 amino acids in length, comprising 14 PPR domains and with a putative mitochondrial targeting signal. Sequence alignment revealed an InDel 26 bp in length and two point mutations between RsRf3-3 and RsRf3-4. Compared with RsRf3-4, the deletion changed the reading frame, resulting in a truncated amino acid sequence in RsRf3-3. A crossing test showed that the RsRf3-3 allele did not restore fertility to CMS in radish. In contrast, the RsRf3-4 allele cosegregated with fertility restoration to CMS in a segregation population. These results strongly suggest that the InDel at the RsRf3 locus could involve transition to fertility in CMS radish. |
| 资助项目 | National Natural Science Foundation of China[31100231] ; National Natural Science Foundation of China[31470412] ; Natural Science Foundation of Hubei Province, China[2007ABA004] ; Natural Science Foundation of Hubei Province, China[2009CDB221] ; Natural Science Foundation of Hubei Province, China[2011CDB408] ; Youth Chenguang Project of Science and Technology of Wuhan, China[200850731402] ; CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture[Y152081t01] |
| WOS研究方向 | Agriculture ; Plant Sciences ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000354407200007 |
| 出版者 | SPRINGER |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/278]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Wang, Ting |
| 作者单位 | 1.Hubei Acad Agr Sci, Wuhan 430064, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Ting,Zhou, Yuan,Gao, Lei,et al. An insertion-deletion at a pentatricopeptide repeat locus linked to fertility transition to cytoplasmic male sterility in radish (Raphanus sativus L.)[J]. MOLECULAR BREEDING,2015,35(4):5. |
| APA | Wang, Ting,Zhou, Yuan,Gao, Lei,Mei, Shi Yong,Wang, Chuan De,&Wang, Zhi Wei.(2015).An insertion-deletion at a pentatricopeptide repeat locus linked to fertility transition to cytoplasmic male sterility in radish (Raphanus sativus L.).MOLECULAR BREEDING,35(4),5. |
| MLA | Wang, Ting,et al."An insertion-deletion at a pentatricopeptide repeat locus linked to fertility transition to cytoplasmic male sterility in radish (Raphanus sativus L.)".MOLECULAR BREEDING 35.4(2015):5. |
入库方式： OAI收割
来源：武汉植物园
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