An RNA sequencing transcriptome analysis of the high-temperature stressed tall fescue reveals novel insights into plant thermotolerance
文献类型：期刊论文
| 作者 | Hu,Tao1; Sun,Xiaoyan1; Zhang,Xunzhong2; Nevo,Eviatar3; Fu,Jinmin1 |
| 刊名 | BMC Genomics
 |
| 出版日期 | 2014-12-19 |
| 卷号 | 15期号:1 |
| 关键词 | High-temperature stress RNA sequencing Cell cycle Energy metabolism Tall fescue |
| ISSN号 | 1471-2164 |
| DOI | 10.1186/1471-2164-15-1147 |
| 英文摘要 | AbstractBackgroundTall fescue (Festuca arundinacea Schreb.) is major cool-season forage and turf grass species worldwide, but high-temperature is a major environmental stress that dramatically threaten forage production and turf management of tall fescue. However, very little is known about the whole-genome molecular mechanisms contributing to thermotolerance. The objectives of this study were to analyzed genome-wide gene expression profiles in the leaves of two tall fescue genotypes, heat tolerant ‘PI578718’ and heat sensitive ‘PI234881’ using high-throughput RNA sequencing.ResultsA total of 262 million high-quality paired-end reads were generated and assembled into 31,803 unigenes with an average length of 1,840?bp. Of these, 12,974 unigenes showed different expression patterns in response to heat stress and were categorized into 49 Gene Ontology functional subcategories. In addition, the variance of enrichment degree in each functional subcategory between PI578718 and PI234881 increased with increasing treatment time. Cell division and cell cycle genes showed a massive increase in transcript abundance in heat-stressed plants and more activated genes were detected in PI 578718 by Kyoto Encyclopedia of Genes and Genomes pathways analysis. Low molecular weight heat shock protein (LMW-HSP, HSP20) showed activated in two stressed genotypes and high molecular weight HSP (HMW-HSP, HSP90) just in PI578718. Assimilation such as photosynthesis, carbon fixation, CH4, N, S metabolism decreased along with increased dissimilation such as oxidative phosphorylation.ConclusionsThe assembled transcriptome of tall fescue could serve as a global description of expressed genes and provide more molecular resources for future functional characterization analysis of genomics in cool-season turfgrass in response to high-temperature. Increased cell division, LMW/HMW-HSP, dissimilation and antioxidant transcript amounts in tall fescue were correlated with successful resistance to high temperature stress. |
| 语种 | 英语 |
| WOS记录号 | BMC:10.1186/1471-2164-15-1147 |
| 出版者 | BioMed Central |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/344]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Nevo,Eviatar; Fu,Jinmin |
| 作者单位 | 1.Wuhan Botanical Garden, Chinese Academy of Science; Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture 2.Virginia Polytechnic Institute and State University; Department of Crop and Soil Environmental Sciences 3.University of Haifa; Institute of Evolution and the International Graduate Center of Evolution |
| 推荐引用方式 GB/T 7714 | Hu,Tao,Sun,Xiaoyan,Zhang,Xunzhong,et al. An RNA sequencing transcriptome analysis of the high-temperature stressed tall fescue reveals novel insights into plant thermotolerance[J]. BMC Genomics,2014,15(1). |
| APA | Hu,Tao,Sun,Xiaoyan,Zhang,Xunzhong,Nevo,Eviatar,&Fu,Jinmin.(2014).An RNA sequencing transcriptome analysis of the high-temperature stressed tall fescue reveals novel insights into plant thermotolerance.BMC Genomics,15(1). |
| MLA | Hu,Tao,et al."An RNA sequencing transcriptome analysis of the high-temperature stressed tall fescue reveals novel insights into plant thermotolerance".BMC Genomics 15.1(2014). |
入库方式： OAI收割
来源：武汉植物园
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