Antisense-mediated FLC transcriptional repression requires the P-TEFb transcription elongation factor
文献类型：期刊论文
| 作者 | Wang, Zhi-Wei; Wu, Zhe; Raitskin, Oleg; Sun, Qianwen; Dean, Caroline |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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| 出版日期 | 2014 |
| 英文摘要 | The functional significance of noncoding transcripts is currently a major question in biology. We have been studying the function of a set of antisense transcripts called COOLAIR that encompass the whole transcription unit of the Arabidopsis floral repressor FLOWERING LOCUS C (FLC). Alternative polyadenylation of COOLAIR transcripts correlates with different FLC sense expression states. Suppressor mutagenesis aimed at understanding the importance of this sense-antisense transcriptional circuitry has identified a role for Arabidopsis cyclin-dependent kinase C (CDKC; 2) in FLC repression. CDKC; 2 functions in an Arabidopsis positive transcription elongation factor b (P-TEFb) complex and influences global RNA polymerase II (Pol II) Ser(2) phosphorylation levels. CDKC; 2 activity directly promotes COOLAIR transcription but does not affect an FLC transgene missing the COOLAIR promoter. In the endogenous gene context, however, the reduction of COOLAIR transcription by cdkc; 2 disrupts a COOLAIR-mediated repression mechanism that increases FLC expression. This disruption then feeds back to indirectly increase COOLAIR expression. This tight interconnection between sense and antisense transcription, together with differential promoter sensitivity to P-TEFb, is central to quantitative regulation of this important floral repressor gene. |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/436]  |
| 专题 | 中国科学院武汉植物园 |
| 推荐引用方式 GB/T 7714 | Wang, Zhi-Wei,Wu, Zhe,Raitskin, Oleg,et al. Antisense-mediated FLC transcriptional repression requires the P-TEFb transcription elongation factor[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2014. |
| APA | Wang, Zhi-Wei,Wu, Zhe,Raitskin, Oleg,Sun, Qianwen,&Dean, Caroline.(2014).Antisense-mediated FLC transcriptional repression requires the P-TEFb transcription elongation factor.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. |
| MLA | Wang, Zhi-Wei,et al."Antisense-mediated FLC transcriptional repression requires the P-TEFb transcription elongation factor".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2014). |
入库方式： OAI收割
来源：武汉植物园
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