The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacer
文献类型：期刊论文
| 作者 | Bian, Yin-bing; Zhong, Cai-hong; Li, Li |
| 刊名 | ELECTRONIC JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2014 |
| 英文摘要 | Background: For the crossbreeding of Auricularia auricula-judae, selecting the appropriated parents in hybridization is very important. However, the classification and diversity analysis of A. auricula-judae has been equivocal, due to the similarity of the fruiting body morphology and its susceptibility to environmental influences. For this purpose, the molecular diversity of 32 A. auricula-judae commercial cultivars in China was analyzed by using the nuclear ribosomal DNA intergenic spacer. Results: The complete nuclear rDNA gene complex of A. auricula-judae isolate is 11,210 bp long, and contains the 18S, 5.8S, and 28S rRNA gene as well as the ITS and IGS regions. Based on the sequence data, four more effective primer combinations for the IGS region of A. auricula-judae were designed. Nucleotide sequence variation in the IGS among 32 A. auricula-judae commercial cultivars in China sorted into three strongly supported clades, which is correlated with geographical regions. Most strains originated from the same area were with a narrow genetic basis and could possibly be domesticated from the local wild-type strains. Conclusion: The grouping information obtained in the present work provides significant information for further genetic improvement in A. auricula-judae, and suggested that the IGS region can be used as an excellent tool for identification of genetic variation. (C) 2014 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B. V. All rights reserved. |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/493]  |
| 专题 | 中国科学院武汉植物园 |
| 推荐引用方式 GB/T 7714 | Bian, Yin-bing,Zhong, Cai-hong,Li, Li. The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacer[J]. ELECTRONIC JOURNAL OF BIOTECHNOLOGY,2014. |
| APA | Bian, Yin-bing,Zhong, Cai-hong,&Li, Li.(2014).The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacer.ELECTRONIC JOURNAL OF BIOTECHNOLOGY. |
| MLA | Bian, Yin-bing,et al."The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacer".ELECTRONIC JOURNAL OF BIOTECHNOLOGY (2014). |
入库方式： OAI收割
来源：武汉植物园
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