Construction of an integrated enzyme system consisting azoreductase and glucose 1-dehydrogenase for dye removal
文献类型：期刊论文
| 作者 | Yang, Yuyi; Wei, Buqing; Zhao, Yuhua; Wang, Jun |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2013 |
| 英文摘要 | Azo dyes are toxic and carcinogenic and are often present in industrial effluents. In this research, azoreductase and glucose 1-dehydrogenase were coupled for both continuous generation of the cofactor NADH and azo dye removal. The results show that 85% maximum relative activity of azoreductase in an integrated enzyme system was obtained at the conditions: 1 U azoreductase:10 U glucose 1-dehydrogenase, 250 mM glucose, 1.0 mM NAD(+) and 150 mu M methyl red. Sensitivity analysis of the factors in the enzyme system affecting dye removal examined by an artificial neural network model shows that the relative importance of enzyme ratio between azoreductase and glucose 1-dehydrogenase was 22%, followed by dye concentration (27%), NAD(+) concentration (23%) and glucose concentration (22%), indicating none of the variables could be ignored in the enzyme system. Batch results show that the enzyme system has application potential for dye removal. (C) 2012 Elsevier Ltd. All rights reserved. |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/671]  |
| 专题 | 中国科学院武汉植物园 |
| 推荐引用方式 GB/T 7714 | Yang, Yuyi,Wei, Buqing,Zhao, Yuhua,et al. Construction of an integrated enzyme system consisting azoreductase and glucose 1-dehydrogenase for dye removal[J]. BIORESOURCE TECHNOLOGY,2013. |
| APA | Yang, Yuyi,Wei, Buqing,Zhao, Yuhua,&Wang, Jun.(2013).Construction of an integrated enzyme system consisting azoreductase and glucose 1-dehydrogenase for dye removal.BIORESOURCE TECHNOLOGY. |
| MLA | Yang, Yuyi,et al."Construction of an integrated enzyme system consisting azoreductase and glucose 1-dehydrogenase for dye removal".BIORESOURCE TECHNOLOGY (2013). |
入库方式： OAI收割
来源：武汉植物园
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