Integration of phosphoproteomic, chemical, and biological strategies for the functional analysis of targeted protein phosphorylation
文献类型：期刊论文
| 作者 | Guo, Mingquan; Huang, Bill X. |
| 刊名 | PROTEOMICS
 |
| 出版日期 | 2013 |
| 英文摘要 | Reversible phosphorylation, tightly controlled by protein kinases and phosphatases, plays a central role in mediating biological processes, such as proteinprotein interactions, subcellular translocation, and activation of cellular enzymes. MS-based phosphoproteomics has now allowed the detection and quantification of tens of thousands of phosphorylation sites from a typical biological sample in a single experiment, which has posed new challenges in functional analysis of each and every phosphorylation site on specific signaling phosphoproteins of interest. In this article, we review recent advances in the functional analysis of targeted phosphorylation carried out by various chemical and biological approaches in combination with the MS-based phosphoproteomics. This review focuses on three types of strategies, including forward functional analysis, defined for the result-driven phosphoproteomics efforts in determining the substrates of a specific protein kinase; reverse functional analysis, defined for tracking the kinase(s) for specific phosphosite(s) derived from the discovery-driven phosphoproteomics efforts; and MS-based analysis on the structurefunction relationship of phosphoproteins. It is expected that this review will provide a state-of-the-art overview of functional analysis of site-specific phosphorylation and explore new perspectives and outline future challenges. |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/673]  |
| 专题 | 中国科学院武汉植物园 |
| 推荐引用方式 GB/T 7714 | Guo, Mingquan,Huang, Bill X.. Integration of phosphoproteomic, chemical, and biological strategies for the functional analysis of targeted protein phosphorylation[J]. PROTEOMICS,2013. |
| APA | Guo, Mingquan,&Huang, Bill X..(2013).Integration of phosphoproteomic, chemical, and biological strategies for the functional analysis of targeted protein phosphorylation.PROTEOMICS. |
| MLA | Guo, Mingquan,et al."Integration of phosphoproteomic, chemical, and biological strategies for the functional analysis of targeted protein phosphorylation".PROTEOMICS (2013). |
入库方式： OAI收割
来源：武汉植物园
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