Site-specific deletions in the tomato genome by the CinH-RS2 and ParA-MRS recombination systems
文献类型：期刊论文
| 作者 | Ow, David W.; Yau, Yuan-Yeu; Zhou, Yin; Wang, Ying |
| 刊名 | PLANT BIOTECHNOLOGY REPORTS
 |
| 出版日期 | 2012 |
| 英文摘要 | We have tested the CinH-RS2 and ParA-MRS site-specific deletion systems in tomato (Solanum lycopersicum L.). The ParA-MRS system is derived from the broad-host-range plasmid RK2, where the 222 aa ParA recombinase recognizes a 133 bp multimer resolution site (MRS). The CinH-RS2 system is derived from Acinetobacter plasmids pKLH2 and pKLH204, where the 188 amino acid CinH recombinase recognizes a 113-bp recombination site known as RS2. In this study, target lines containing a DNA segment flanked by recombination sites were crossed to recombinase-expressing lines producing CinH or ParA recombinase. CinH-mediated recombination of RS2 substrates was detected in 2 of 3 F-1 plants that harbor both the target and recombinase loci. On the other hand, recombination mediated by ParA was not detected among F-1 plants, but was found among 13 of 47 F-2 plants. These data show that both systems can mediate site-specific DNA deletion in the tomato genome, and, upon further refinement, can provide additional molecular tools for tomato improvement through precise genome manipulation. As the target construct also contains additional recombination sites for site-specific integration by other recombination systems, these tomato lines could be used for future testing of gene stacking through site-specific integration. |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/759]  |
| 专题 | 中国科学院武汉植物园 |
| 推荐引用方式 GB/T 7714 | Ow, David W.,Yau, Yuan-Yeu,Zhou, Yin,et al. Site-specific deletions in the tomato genome by the CinH-RS2 and ParA-MRS recombination systems[J]. PLANT BIOTECHNOLOGY REPORTS,2012. |
| APA | Ow, David W.,Yau, Yuan-Yeu,Zhou, Yin,&Wang, Ying.(2012).Site-specific deletions in the tomato genome by the CinH-RS2 and ParA-MRS recombination systems.PLANT BIOTECHNOLOGY REPORTS. |
| MLA | Ow, David W.,et al."Site-specific deletions in the tomato genome by the CinH-RS2 and ParA-MRS recombination systems".PLANT BIOTECHNOLOGY REPORTS (2012). |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。