Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically
文献类型：期刊论文
| 作者 | Geng Yahong; Li Yeguang; Zhang Chengwu; Zeng Mingtao; Lu Dayan; Miao Fengping |
| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2012 |
| 英文摘要 | The influence of alkali on astaxanthin and the optimal working wave length for measurement of astaxanthin from Haematococcus crude extract were investigated, and a spectrophotometric method for precise quantification of the astaxanthin based on the method of Boussiba et al. was established. According to Boussiba's method, alkali treatment destroys chlorophyll. However, we found that: 1) carotenoid content declined for about 25% in Haematococcus fresh cysts and up to 30% in dry powder of Haematococcus broken cysts after alkali treatment; and 2) dimethyl sulfoxide (DMSO)-extracted chlorophyll of green Haematococcus bares little absorption at 520-550 nm. Interestingly, a good linear relationship existed between absorbance at 530 nm and astaxanthin content, while an unknown interference at 540-550 nm was detected in our study. Therefore, with 530 nm as working wavelength, the alkali treatment to destroy chlorophyll was not necessary and the influence of chlorophyll, other carotenoids, and the unknown interference could be avoided. The astaxanthin contents of two samples were measured at 492 nm and 530 nm; the measured values at 530 nm were 2.617 g/100 g and 1.811 g/100 g. When compared with the measured values at 492 nm, the measured values at 530 nm decreased by 6.93% and 11.96%, respectively. The measured values at 530 nm are closer to the true astaxanthin contents in the samples. The data show that 530 nm is the most suitable wave length for spectrophotometric determination to the astaxanthin in Haematococcus crude extract. |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/760]  |
| 专题 | 中国科学院武汉植物园 |
| 推荐引用方式 GB/T 7714 | Geng Yahong,Li Yeguang,Zhang Chengwu,et al. Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2012. |
| APA | Geng Yahong,Li Yeguang,Zhang Chengwu,Zeng Mingtao,Lu Dayan,&Miao Fengping.(2012).Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY. |
| MLA | Geng Yahong,et al."Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2012). |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。