MICROSATELLITE PRIMERS IN THE ENDANGERED QUILLWORT ISOETES HYPSOPHILA (ISOETACEAE) AND CROSS-AMPLIFICATION IN I. SINENSIS
文献类型:期刊论文
作者 | Li, Zuozhou; Li, Wei; Chen, Yuanyuan; Han, Qingxiang |
刊名 | AMERICAN JOURNAL OF BOTANY
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出版日期 | 2012 |
英文摘要 | Premise of the study: The first microsatellite primers were developed for Isoetes hypsophila, an endangered quillwort species endemic to the Qinghai-Tibetan Plateau in China, to further describe its genetic variability and population structure. We also examined their cross-amplification in a congeneric species, I. sinensis. Methods and Results: Using the Fast Isolation by AFLP of Sequences COntaining Repeats (FIASCO) protocol, nine microsatellite loci were isolated and characterized in 32 samples from four natural populations of I. hypsophila. The primers amplified di- and hexanucleotide repeats with three to 11 alleles per locus. Seven of nine primers were cross-amplified in I. sinensis with two to seven alleles per locus. Conclusion: The microsatellite loci primers will be useful for studies of genetic diversity and gene flow in natural populations of Isoetes species. |
源URL | [http://202.127.146.157/handle/2RYDP1HH/776] ![]() |
专题 | 中国科学院武汉植物园 |
推荐引用方式 GB/T 7714 | Li, Zuozhou,Li, Wei,Chen, Yuanyuan,et al. MICROSATELLITE PRIMERS IN THE ENDANGERED QUILLWORT ISOETES HYPSOPHILA (ISOETACEAE) AND CROSS-AMPLIFICATION IN I. SINENSIS[J]. AMERICAN JOURNAL OF BOTANY,2012. |
APA | Li, Zuozhou,Li, Wei,Chen, Yuanyuan,&Han, Qingxiang.(2012).MICROSATELLITE PRIMERS IN THE ENDANGERED QUILLWORT ISOETES HYPSOPHILA (ISOETACEAE) AND CROSS-AMPLIFICATION IN I. SINENSIS.AMERICAN JOURNAL OF BOTANY. |
MLA | Li, Zuozhou,et al."MICROSATELLITE PRIMERS IN THE ENDANGERED QUILLWORT ISOETES HYPSOPHILA (ISOETACEAE) AND CROSS-AMPLIFICATION IN I. SINENSIS".AMERICAN JOURNAL OF BOTANY (2012). |
入库方式: OAI收割
来源:武汉植物园
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