A high-throughput apple SNP genotyping platform using the GoldenGate (TM) assay
文献类型：期刊论文
| 作者 | Khan, M. Awais; Han, Yuepeng; Zhao, Youfu Frank; Korban, Schuyler S. |
| 刊名 | GENE
 |
| 出版日期 | 2012 |
| 英文摘要 | EST data generated from 14 apple genotypes were downloaded from NCBI and mapped against a reference EST assembly to identify Single Nucleotide Polymorphisms (SNPs). Mapping of these SNPs was undertaken using 90% of sequence similarity and minimum coverage of four reads at each SNP position. In total, 37,807 SNPs were identified with an average of one SNP every 187 bp from a total of 6888 unique EST contigs. Identified SNPs were checked for flanking sequences of >= 60 bp along both sides of SNP alleles for reliable design of a custom high-throughput genotyping assay. A total of 12,299 SNPs, representing 6525 contigs, fit the selected criterion of >= 60 bp sequences flanking a SNP position. Of these, 1411 SNPs were validated using four apple genotypes. Based on genotyping assays, it was estimated that 60% of SNPs were valid SNPs, while 26% of SNPs might be derived from paralogous regions. (C) 2011 Elsevier B.V. All rights reserved. |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/796]  |
| 专题 | 中国科学院武汉植物园 |
| 推荐引用方式 GB/T 7714 | Khan, M. Awais,Han, Yuepeng,Zhao, Youfu Frank,et al. A high-throughput apple SNP genotyping platform using the GoldenGate (TM) assay[J]. GENE,2012. |
| APA | Khan, M. Awais,Han, Yuepeng,Zhao, Youfu Frank,&Korban, Schuyler S..(2012).A high-throughput apple SNP genotyping platform using the GoldenGate (TM) assay.GENE. |
| MLA | Khan, M. Awais,et al."A high-throughput apple SNP genotyping platform using the GoldenGate (TM) assay".GENE (2012). |
入库方式： OAI收割
来源：武汉植物园
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