Integration of physical and genetic maps in apple confirms whole-genome and segmental duplications in the apple genome
文献类型：期刊论文
| 作者 | Han, Yuepeng2; Zheng, Danman1; Vimolmangkang, Sornkanok1; Khan, Muhammad A.1; Beever, Jonathan E.3; Korban, Schuyler S.1 |
| 刊名 | JOURNAL OF EXPERIMENTAL BOTANY
 |
| 出版日期 | 2011-10-01 |
| 卷号 | 62期号:14页码:5117-5130 |
| 关键词 | Genetic map genome duplication Malusxdomestica physical map segmental duplication simple sequence repeat |
| ISSN号 | 0022-0957 |
| DOI | 10.1093/jxb/err215 |
| 英文摘要 | A total of 355 simple sequence repeat (SSR) markers were developed, based on expressed sequence tag (EST) and bacterial artificial chromosome (BAC)-end sequence databases, and successfully used to construct an SSR-based genetic linkage map of the apple. The consensus linkage map spanned 1143 cM, with an average density of 2.5 cM per marker. Newly developed SSR markers along with 279 SSR markers previously published by the HiDRAS project were further used to integrate physical and genetic maps of the apple using a PCR-based BAC library screening approach. A total of 470 contigs were unambiguously anchored onto all 17 linkage groups of the apple genome, and 158 contigs contained two or more molecular markers. The genetically mapped contigs spanned similar to 421 Mb in cumulative physical length, representing 60.0% of the genome. The sizes of anchored contigs ranged from 97 kb to 4.0 Mb, with an average of 995 kb. The average physical length of anchored contigs on each linkage group was similar to 24.8 Mb, ranging from 17.0 Mb to 37.73 Mb. Using BAC DNA as templates, PCR screening of the BAC library amplified fragments of highly homologous sequences from homoeologous chromosomes. Upon integrating physical and genetic maps of the apple, the presence of not only homoeologous chromosome pairs, but also of multiple locus markers mapped to adjacent sites on the same chromosome was detected. These findings demonstrated the presence of both genome-wide and segmental duplications in the apple genome and provided further insights into the complex polyploid ancestral origin of the apple. |
| 资助项目 | USDA Cooperative State Research, Education and Extension Service-National Research Initiative[2005-35300-15538] ; Illinois C-FAR grant[06I-003-3-SEN] ; National Natural Science Foundation of China[30971987] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000295983800033 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/842]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Korban, Schuyler S. |
| 作者单位 | 1.Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 3.Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA |
| 推荐引用方式 GB/T 7714 | Han, Yuepeng,Zheng, Danman,Vimolmangkang, Sornkanok,et al. Integration of physical and genetic maps in apple confirms whole-genome and segmental duplications in the apple genome[J]. JOURNAL OF EXPERIMENTAL BOTANY,2011,62(14):5117-5130. |
| APA | Han, Yuepeng,Zheng, Danman,Vimolmangkang, Sornkanok,Khan, Muhammad A.,Beever, Jonathan E.,&Korban, Schuyler S..(2011).Integration of physical and genetic maps in apple confirms whole-genome and segmental duplications in the apple genome.JOURNAL OF EXPERIMENTAL BOTANY,62(14),5117-5130. |
| MLA | Han, Yuepeng,et al."Integration of physical and genetic maps in apple confirms whole-genome and segmental duplications in the apple genome".JOURNAL OF EXPERIMENTAL BOTANY 62.14(2011):5117-5130. |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。