Gene Expression Profile Changes in Germinating Rice
文献类型：期刊论文
| 作者 | He, Dongli1; Han, Chao1,2; Yang, Pingfang1 |
| 刊名 | JOURNAL OF INTEGRATIVE PLANT BIOLOGY
 |
| 出版日期 | 2011-10-01 |
| 卷号 | 53期号:10页码:835-844 |
| 关键词 | rice seed two-dimensional gel electrophoresis matrix assisted laser desorption ionization-time of flight semi-quantitative real time polymerase chain reaction germination proteomics imbibition |
| ISSN号 | 1672-9072 |
| DOI | 10.1111/j.1744-7909.2011.01074.x |
| 英文摘要 | Water absorption is a prerequisite for seed germination. During imbibition, water influx causes the resumption of many physiological and metabolic processes in growing seed. In order to obtain more complete knowledge about the mechanism of seed germination, two-dimensional gel electrophoresis was applied to investigate the protein profile changes of rice seed during the first 48 h of imbibition. Thirty-nine differentially expressed proteins were identified, including 19 down-regulated and 20 up-regulated proteins. Storage proteins and some seed development-and desiccation-associated proteins were down regulated. The changed patterns of these proteins indicated extensive mobilization of seed reserves. By contrast, catabolism-associated proteins were up regulated upon imbibition. Semi-quantitative real time polymerase chain reaction analysis showed that most of the genes encoding the down- or u-pregulated proteins were also down or up regulated at mRNA level. The expression of these genes was largely consistent at mRNA and protein levels. In providing additional information concerning gene regulation in early plant life, this study will facilitate understanding of the molecular mechanisms of seed germination. |
| 资助项目 | Chinese Academy of Sciences |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000295879700006 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/845]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Yang, Pingfang |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Dongli,Han, Chao,Yang, Pingfang. Gene Expression Profile Changes in Germinating Rice[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2011,53(10):835-844. |
| APA | He, Dongli,Han, Chao,&Yang, Pingfang.(2011).Gene Expression Profile Changes in Germinating Rice.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,53(10),835-844. |
| MLA | He, Dongli,et al."Gene Expression Profile Changes in Germinating Rice".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 53.10(2011):835-844. |
入库方式： OAI收割
来源：武汉植物园
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