DEVELOPMENT OF MICROSATELLITE LOCI FOR CEPHALOTAXUS OLIVERI (CEPHALOTAXACEAE) AND CROSS-AMPLIFICATION IN CEPHALOTAXUS
文献类型：期刊论文
| 作者 | Pan, Hua-Wei1; Guo, Ying-Rong2; Su, Ying-Juan1; Wang, Ting3 |
| 刊名 | AMERICAN JOURNAL OF BOTANY
 |
| 出版日期 | 2011-08-01 |
| 卷号 | 98期号:8页码:E229-E232 |
| 关键词 | Cephalotaxus oliveri cross-species amplification genetic diversity microsatellite loci population differentiation |
| ISSN号 | 0002-9122 |
| DOI | 10.3732/ajb.1100128 |
| 英文摘要 | Premise of the study: Microsatellite loci were developed for Cephalotaxus oliveri, an endemic and endangered conifer in China, which will allow assessment of the levels of genetic diversity and a means to understand the genetic consequences of habitat fragmentation. Methods and Results: Using the Fast Isolation by AFLP of Sequences COntaining (FIASCO) Repeats protocol, 19 microsatellite loci were identified in C. oliveri, 13 of which were polymorphic within a sample of 52 individuals representing five natural populations. The actual number of alleles per locus ranged from one to five. Twelve polymorphic loci were also successfully amplified in C. fortunei. Conclusions: These microsatellite loci will provide a useful tool for further investigating genetic variation in natural populations of C. oliveri, which will inform future conservation and management strategies. Additionally, cross-amplification in C. fortunei suggested the potential utility of these loci in this and other congeneric species. |
| 资助项目 | National Natural Science Foundation of China[30170789] ; National Natural Science Foundation of China[30270153] ; National Natural Science Foundation of China[30771763] ; National Natural Science Foundation of China[30970290] ; National Natural Science Foundation of China[31070594] ; Chinese Academy of Sciences[KSCX2-YW-Z-0940] ; Sun Yat-sen University |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000293513400011 |
| 出版者 | BOTANICAL SOC AMER INC |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/860]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Su, Ying-Juan |
| 作者单位 | 1.Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China 2.Jiangxi Wildlife Protect & Management Adm, Nanchang 330038, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan Bot Garden, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pan, Hua-Wei,Guo, Ying-Rong,Su, Ying-Juan,et al. DEVELOPMENT OF MICROSATELLITE LOCI FOR CEPHALOTAXUS OLIVERI (CEPHALOTAXACEAE) AND CROSS-AMPLIFICATION IN CEPHALOTAXUS[J]. AMERICAN JOURNAL OF BOTANY,2011,98(8):E229-E232. |
| APA | Pan, Hua-Wei,Guo, Ying-Rong,Su, Ying-Juan,&Wang, Ting.(2011).DEVELOPMENT OF MICROSATELLITE LOCI FOR CEPHALOTAXUS OLIVERI (CEPHALOTAXACEAE) AND CROSS-AMPLIFICATION IN CEPHALOTAXUS.AMERICAN JOURNAL OF BOTANY,98(8),E229-E232. |
| MLA | Pan, Hua-Wei,et al."DEVELOPMENT OF MICROSATELLITE LOCI FOR CEPHALOTAXUS OLIVERI (CEPHALOTAXACEAE) AND CROSS-AMPLIFICATION IN CEPHALOTAXUS".AMERICAN JOURNAL OF BOTANY 98.8(2011):E229-E232. |
入库方式： OAI收割
来源：武汉植物园
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