中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
CHARACTERIZATION OF 15 NOVEL SINGLE NUCLEOTIDE POLYMORPHISMS (SNPS) IN THE ACTINIDIA CHINENSIS SPECIES COMPLEX (ACTINIDIACEAE)

文献类型:期刊论文

作者Zhou, Jin2,3; Liu, Yifei1; Huang, Hongwen1
刊名AMERICAN JOURNAL OF BOTANY
出版日期2011-05-01
卷号98期号:5页码:E100-E102
关键词Actinidia Cleaved Amplified polymorphic Sequences (CAPS) Expressed Sequence Tags (EST) Single Nucleotide Polymorphism (SNP)
ISSN号0002-9122
DOI10.3732/ajb.1000442
英文摘要. Premise of the study : Based on the ESTs database, we report here for the first time 15 polymorphic single nucleotide polymorphisms (SNPs) in the Actinidia chinensis species complex that can be used to study population genetic variation. . Methods and Results : SNPs derived from ESTs of the A. chinensis species complex were identified by the in silico SNP discovery strategy. The Cleaved Amplified polymorphic Sequences (CAPS) method was used to validate putative SNPs in a sample of 24 diploid A. chinensis individuals. For the 15 nuclear SNPs identified, all loci had two alleles, and the minor allele frequency ranged from 0.146 to 0.479. The expected and observed heterozygosities ranged from 0 to 0.500 and from 0 to 0.875, respectively. . Conclusions : The developed markers in this study will provide valuable tools for characterizing genetic variation in evolutionary and ecological studies of natural populations.
资助项目National Natural Science Foundation of China[30900119] ; National Natural Science Foundation of China[30771479] ; Chinese Academy of Sciences[KSCX2-YW-N-061]
WOS研究方向Plant Sciences
语种英语
WOS记录号WOS:000290480600001
出版者BOTANICAL SOC AMER INC
源URL[http://202.127.146.157/handle/2RYDP1HH/873]  
专题中国科学院武汉植物园
通讯作者Huang, Hongwen
作者单位1.Chinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R China
2.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
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Zhou, Jin,Liu, Yifei,Huang, Hongwen. CHARACTERIZATION OF 15 NOVEL SINGLE NUCLEOTIDE POLYMORPHISMS (SNPS) IN THE ACTINIDIA CHINENSIS SPECIES COMPLEX (ACTINIDIACEAE)[J]. AMERICAN JOURNAL OF BOTANY,2011,98(5):E100-E102.
APA Zhou, Jin,Liu, Yifei,&Huang, Hongwen.(2011).CHARACTERIZATION OF 15 NOVEL SINGLE NUCLEOTIDE POLYMORPHISMS (SNPS) IN THE ACTINIDIA CHINENSIS SPECIES COMPLEX (ACTINIDIACEAE).AMERICAN JOURNAL OF BOTANY,98(5),E100-E102.
MLA Zhou, Jin,et al."CHARACTERIZATION OF 15 NOVEL SINGLE NUCLEOTIDE POLYMORPHISMS (SNPS) IN THE ACTINIDIA CHINENSIS SPECIES COMPLEX (ACTINIDIACEAE)".AMERICAN JOURNAL OF BOTANY 98.5(2011):E100-E102.

入库方式: OAI收割

来源:武汉植物园

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