Cloning and Functional Characterization of a Chalcone Isomerase from Trigonella foenum-graecum L.
文献类型：期刊论文
| 作者 | Qin, Jian-chun1; Zhu, Lin1; Gao, Ming-jun2; Wu, Xian1; Pan, Hong-yu1; Zhang, Yan-sheng3; Li, Xiang1,2 |
| 刊名 | PLANTA MEDICA
 |
| 出版日期 | 2011-05-01 |
| 卷号 | 77期号:7页码:765-770 |
| 关键词 | Trigonella foenum-graecum L. Leguminosae chalcone isomerase Arabidopsis tt5 |
| ISSN号 | 0032-0943 |
| DOI | 10.1055/s-0030-1250566 |
| 英文摘要 | Flavonoids belong to a group of plant natural products with variable phenolic structures and play important roles in protection against biotic and abiotic stress. Fenugreek (Trigonella foenum-graecum L.) seeds and stems contain flavonol glycosides and isoflavone derivatives. Up to now, the molecular features of fenugreek flavonoid biosynthesis have not been characterized. Here we present cloning of a cDNA encoding a chalcone isomerase (namely TFGCHI-1) from the leaves of T. foenum-graecum which convert chalcones to flavanones in vitro. Transformation of Arabidopsis loss-of-function tt5 (chi) mutant with a TFGCHI-1 cDNA complemented tt5 and produced higher levels of flavonol glycosides than wild-type Col-0. |
| 资助项目 | Program for New Century Excellent Talents in University[NCE09-0423] ; Ministry of Education of the People's Republic of China ; National Natural Science Foundation of China[NSFC-31000149] ; Key Projects in the National Science & Technology Pillar Program[2006BAD08A08] ; Basic Research Program[20060544] ; Science and Technology Department of Jilin Province ; International Technology Cooperation Project, Changchun, China[06GH07] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000290303200015 |
| 出版者 | GEORG THIEME VERLAG KG |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/874]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Li, Xiang |
| 作者单位 | 1.Jilin Univ, Coll Plant Sci, Changchun 130062, Peoples R China 2.Agr & Agri Food Canada, Saskatoon Res Ctr, Saskatoon, SK, Canada 3.Chinese Acedamy Sci, Wuhan Bot Garden, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, Jian-chun,Zhu, Lin,Gao, Ming-jun,et al. Cloning and Functional Characterization of a Chalcone Isomerase from Trigonella foenum-graecum L.[J]. PLANTA MEDICA,2011,77(7):765-770. |
| APA | Qin, Jian-chun.,Zhu, Lin.,Gao, Ming-jun.,Wu, Xian.,Pan, Hong-yu.,...&Li, Xiang.(2011).Cloning and Functional Characterization of a Chalcone Isomerase from Trigonella foenum-graecum L..PLANTA MEDICA,77(7),765-770. |
| MLA | Qin, Jian-chun,et al."Cloning and Functional Characterization of a Chalcone Isomerase from Trigonella foenum-graecum L.".PLANTA MEDICA 77.7(2011):765-770. |
入库方式： OAI收割
来源：武汉植物园
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