BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes
文献类型：期刊论文
| 作者 | Simkova, Hana1; Safar, Jan1; Kubalakova, Marie1; Suchankova, Pavla1; Cihalikova, Jarmila1; Robert-Quatre, Heda1; Azhaguvel, Perumal2; Weng, Yiqun2,3; Peng, Junhua4,5,6; Lapitan, Nora L. V.4 |
| 刊名 | JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY
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| 出版日期 | 2011 |
| 页码 | 11 |
| ISSN号 | 1110-7243 |
| DOI | 10.1155/2011/302543 |
| 英文摘要 | Positional cloning in bread wheat is a tedious task due to its huge genome size and hexaploid character. BAC libraries represent an essential tool for positional cloning. However, wheat BAC libraries comprise more than million clones, which makes their screening very laborious. Here, we present a targeted approach based on chromosome-specific BAC libraries. Such libraries were constructed from flow-sorted arms of wheat chromosome 7D. A library from the short arm (7DS) consisting of 49,152 clones with 113 kb insert size represented 12.1 arm equivalents whereas a library from the long arm (7DL) comprised 50,304 clones of 116 kb providing 14.9x arm coverage. The 7DS library was PCR screened with markers linked to Russian wheat aphid resistance gene DnCI2401, the 7DL library was screened by hybridization with a probe linked to greenbug resistance gene Gb3. The small number of clones combined with high coverage made the screening highly efficient and cost effective. |
| 资助项目 | Czech Science Foundation[521/07/1573] ; Czech Republic Ministry of Education, Youth and Sports[LC06004] ; National Basic Research Program of China (973 Program)[2009CB118300] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Research & Experimental Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000286232600001 |
| 出版者 | HINDAWI PUBLISHING CORPORATION |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/904]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Simkova, Hana |
| 作者单位 | 1.Inst Expt Bot, Lab Mol Cytogenet & Cytometry, CZ-77200 Olomouc, Czech Republic 2.Texas AgriLife Res, Amarillo, TX 79106 USA 3.Univ Wisconsin, Dept Hort, USDA,ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA 4.Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA 5.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China 6.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 7.Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA |
| 推荐引用方式 GB/T 7714 | Simkova, Hana,Safar, Jan,Kubalakova, Marie,et al. BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes[J]. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY,2011:11. |
| APA | Simkova, Hana.,Safar, Jan.,Kubalakova, Marie.,Suchankova, Pavla.,Cihalikova, Jarmila.,...&Dolezel, Jaroslav.(2011).BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes.JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY,11. |
| MLA | Simkova, Hana,et al."BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes".JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY (2011):11. |
入库方式： OAI收割
来源：武汉植物园
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