Ectopic expression of a grapevine transcription factor VvWRKY11 contributes to osmotic stress tolerance in Arabidopsis
文献类型：期刊论文
| 作者 | Liu, Huaying2,3; Yang, Wenlong4; Liu, Dongcheng4; Han, Yuepeng1; Zhang, Aimin4; Li, Shaohua1 |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2011 |
| 卷号 | 38期号:1页码:417-427 |
| 关键词 | WRKY transcription factor Grapevine Osmotic stress Defense response |
| ISSN号 | 0301-4851 |
| DOI | 10.1007/s11033-010-0124-0 |
| 英文摘要 | Plant WRKY transcriptional factors play an important role in response to biotic and abiotic stresses. In this study, a WRKY transcription factor was isolated from grapevine. This transcription factor showed 66% and 58% identity at the DNA and amino acid sequence levels, respectively, with Arabidopsis AtWRKY11 genes, and was therefore designated VvWRKY11. Phylogenetic analysis and structure comparison indicated that VvWRKY11 protein belongs to group IIc. The VvWRKY11 protein was shown to be located in the nucleus based on green fluorescent protein analysis. Yeast one-hybrid analysis further indicated that VvWRKY11 protein binds specifically to the W-box element. The expression profile of VvWRKY11 in response to treatment with phytohormone salicylic acid or pathogen Plasmopara viticola is rapid and transient. Transgenic Arabidopsis seedlings overexpressing VvWRKY11 showed higher tolerance to water stress induced by mannitol than wild-type plants. These results clearly demonstrated that the VvWRKY11 gene is involved in the response to dehydration stress. In addition, the role of VvWRKY11 protein in regulating the expression of two stress response genes, AtRD29A and AtRD29B, is also discussed. |
| 资助项目 | Chinese Academy of Sciences[KSCX2-YW-N-032] ; CAS/SAFEA |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000284896200055 |
| 出版者 | SPRINGER |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/906]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Li, Shaohua |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan Bot Garden, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Huaying,Yang, Wenlong,Liu, Dongcheng,et al. Ectopic expression of a grapevine transcription factor VvWRKY11 contributes to osmotic stress tolerance in Arabidopsis[J]. MOLECULAR BIOLOGY REPORTS,2011,38(1):417-427. |
| APA | Liu, Huaying,Yang, Wenlong,Liu, Dongcheng,Han, Yuepeng,Zhang, Aimin,&Li, Shaohua.(2011).Ectopic expression of a grapevine transcription factor VvWRKY11 contributes to osmotic stress tolerance in Arabidopsis.MOLECULAR BIOLOGY REPORTS,38(1),417-427. |
| MLA | Liu, Huaying,et al."Ectopic expression of a grapevine transcription factor VvWRKY11 contributes to osmotic stress tolerance in Arabidopsis".MOLECULAR BIOLOGY REPORTS 38.1(2011):417-427. |
入库方式： OAI收割
来源：武汉植物园
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