A chimeric Rfo gene generated by intergenic recombination cosegregates with the fertility restorer phenotype for cytoplasmic male sterility in radish
文献类型：期刊论文
| 作者 | Wang, Ting2; Wang, Zhi Wei2,3; Zhang, Li Jun1,2; Chen, Jie1,2; Xiang, Chang Ping3; Mei, Shi Yong4; Zhou, Yuan2 |
| 刊名 | MOLECULAR BREEDING
 |
| 出版日期 | 2010-02-01 |
| 卷号 | 25期号:2页码:339-349 |
| 关键词 | Cosegregate Cytoplasmic male sterility Fertility restorer Intergenic recombination Radish |
| ISSN号 | 1380-3743 |
| DOI | 10.1007/s11032-009-9321-8 |
| 英文摘要 | In this work, we have identified a chimeric pentatricopeptide repeat (PPR)-encoding gene cosegregating with the fertility restorer phenotype for cytoplasmic male sterility (CMS) in radish. We have constructed a CMS-Rf system consisting of sterile line '9802A2', maintainer line '9802B2' and restorer line '2007H'. F(2) segregating population analysis indicated that male fertility is restored by a single dominant gene in the CMS-Rf system described above. A PPR gene named Rfoc was found in the restorer line '2007H'. It cosegregated with the fertility restorer in the F(2) segregating population which is composed of 613 fertile plants and 187 sterile plants. The Rfoc gene encodes a predicted protein 687 amino acids in length, comprising 16 PPR domains and with a putative mitochondrial targeting signal. Sequence alignment showed that recombination between the 5' region of Rfob (EU163282) and the 3' region of PPR24 (AY285675) resulted in Rfoc, indicating a recent unequal crossing-over event between Rfo and PPR24 loci at a distance of 5.5 kb. The sterile line '9802A2' contains the rfob gene. In the F(2) population, Rfoc and rfob were observed to fit a segregation ratio 1:2:1 showing that Rfoc was allelic to Rfo. Previously we have reported that a fertile line '2006H', which carries the recessive rfob gene, is able to restore the male fertility of CMS line '9802A1' (Wang et al. in Theor Appl Genet 117:313-320, 2008). However, here when conducting a cross between the fertile line '2006H' and CMS line '9802A2, the resulting plants were male sterile, which shows that sterile line '9802A2' possesses a different nuclear background compared to '9802A1'. Based on these results, the genetic model of fertility restoration for radish CMS is also discussed. |
| 资助项目 | Natural Science Foundation of Hubei province, China[2007ABA004] ; Youth Chenguang Project of Science and Technology of Wuhan, China[200850731402] |
| WOS研究方向 | Agriculture ; Plant Sciences ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000273812300013 |
| 出版者 | SPRINGER |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/981]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Wang, Ting |
| 作者单位 | 1.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Key Lab Aquat Bot & Watershed Ecol, Wuhan Bot Garden, Wuhan 430074, Peoples R China 3.Huazhong Agr Univ, Key Lab Minist Educ Hort Plant Biol, Wuhan 430070, Peoples R China 4.Hubei Acad Agr Sci, Wuhan 430064, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Ting,Wang, Zhi Wei,Zhang, Li Jun,et al. A chimeric Rfo gene generated by intergenic recombination cosegregates with the fertility restorer phenotype for cytoplasmic male sterility in radish[J]. MOLECULAR BREEDING,2010,25(2):339-349. |
| APA | Wang, Ting.,Wang, Zhi Wei.,Zhang, Li Jun.,Chen, Jie.,Xiang, Chang Ping.,...&Zhou, Yuan.(2010).A chimeric Rfo gene generated by intergenic recombination cosegregates with the fertility restorer phenotype for cytoplasmic male sterility in radish.MOLECULAR BREEDING,25(2),339-349. |
| MLA | Wang, Ting,et al."A chimeric Rfo gene generated by intergenic recombination cosegregates with the fertility restorer phenotype for cytoplasmic male sterility in radish".MOLECULAR BREEDING 25.2(2010):339-349. |
入库方式： OAI收割
来源：武汉植物园
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