Assessment of Genetic Diversity of Chinese Sand Pear Landraces (Pyrus pyrifolia Nakai) Using Simple Sequence Repeat Markers
文献类型:期刊论文
作者 | Jiang, Zhengwang2; Tang, Feiyan2; Huang, Hongwen1; Hu, Hongju3; Chen, Qiliang3 |
刊名 | HORTSCIENCE |
出版日期 | 2009-06-01 |
卷号 | 44期号:3页码:619-626 |
ISSN号 | 0018-5345 |
关键词 | Pyrus pyrifolia microsatellites landrace genetic diversity sand pear germplasm |
英文摘要 | The sand pear (Pyrus pyrifolia Nakai) is an important fruit crop in China. In this study, simple sequence repeats (SSRs) were used to estimate the level and pattern of genetic diversity among 233 sand pear landraces collected from 10 different geographic regions in China. The results demonstrated that the SSR technique is an effective tool for assessing genetic diversity and the geographic pattern of genetic variation among sand pear landraces of different origins. A total of 184 putative alleles was detected using 14 primer pairs with an average of 13.1 alleles per locus. The mean expected heterozygosity and observed heterozygosity across all loci were 0.705 and 0.671, respectively. High genetic diversity was found in all populations except for that originated from Jiangxi (A(e) = 3.149; H(e) = 0.655), whereas at the regional level, those from central China were less diverse than those from other regions. Analysis of molecular variance showed that most genetic differences resided among landraces within populations. Additionally, un-weighted pair group with arithmetic average clustering and principal component analysis plotting based on Nei's genetic distance revealed distinct gene pools in agreement with geographic distribution. |
资助项目 | CAS[KSCX2-YW-N-061] ; NSFC[30070082] |
WOS研究方向 | Agriculture |
语种 | 英语 |
出版者 | AMER SOC HORTICULTURAL SCIENCE |
WOS记录号 | WOS:000267154900164 |
源URL | [http://202.127.146.157/handle/2RYDP1HH/1034] |
专题 | 中国科学院武汉植物园 |
通讯作者 | Huang, Hongwen |
作者单位 | 1.Chinese Acad Sci, S China Bot Garden, S China Inst Bot, Guangzhou 510650, Guangdong, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Wuhan Inst Bot, Wuhan 430074, Hubei, Peoples R China 3.Hubei Acad Agr Sci, Inst Fruit & Tea, Wuhan 430209, Hubei, Peoples R China |
推荐引用方式 GB/T 7714 | Jiang, Zhengwang,Tang, Feiyan,Huang, Hongwen,et al. Assessment of Genetic Diversity of Chinese Sand Pear Landraces (Pyrus pyrifolia Nakai) Using Simple Sequence Repeat Markers[J]. HORTSCIENCE,2009,44(3):619-626. |
APA | Jiang, Zhengwang,Tang, Feiyan,Huang, Hongwen,Hu, Hongju,&Chen, Qiliang.(2009).Assessment of Genetic Diversity of Chinese Sand Pear Landraces (Pyrus pyrifolia Nakai) Using Simple Sequence Repeat Markers.HORTSCIENCE,44(3),619-626. |
MLA | Jiang, Zhengwang,et al."Assessment of Genetic Diversity of Chinese Sand Pear Landraces (Pyrus pyrifolia Nakai) Using Simple Sequence Repeat Markers".HORTSCIENCE 44.3(2009):619-626. |
入库方式: OAI收割
来源:武汉植物园
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