Molecular Cloning and Sequence Analysis of Cyanovirin-N Homology Gene in Ceratopteris thalictroides
文献类型：期刊论文
| 作者 | Qi, Xiaoqiong1; Yang, Yongxia1; Su, Yingjuan2; Wang, Ting1 |
| 刊名 | AMERICAN FERN JOURNAL
 |
| 出版日期 | 2009-04-01 |
| 卷号 | 99期号:2页码:78-92 |
| 关键词 | Ceratopteris thalictroides chromosome walking single oligonucleotide nested PCR inverse PCR thermal asymmetric interlaced PCR CVNH bioinformatic analysis |
| ISSN号 | 0002-8444 |
| 英文摘要 | A new full-length genomic DNA, encoding a member of the cyanovirin-N (CV-N) homologous protein family, has been cloned from the fern species Gerotopteris thalictroides by chromosome walking. It is 1993 bp long, contains a 723 bp open reading frame (ORF) that encodes a deduced protein (named CtCVNH) with 150 amino acid residues. CtCVNH has a predicted isoelectric point (PI) of 4.47 and a calculated molecular mass 15.9556 kDa. It possesses the conserved anti-HIV (human immunodeficiency virus) CV-N domain, which is the same as the cyanovirin-N homology (CVNH) members that were isolated from filamentous ascomycetes and C. richardii. Modeling of the tertiary structure indicated that CtCVNH is an elongated, largely P-sheet protein that displays internal two-fold pseudosymmetry. Comparative structure analysis of the predicted CtCVNH with native CV-N revealed that the major evolutionary changes occurring during the evolution of plant CVNHs were: 1) a length increase at N- and C-terminal regions; and 2) a loop to helix transition at the helical-turn regions. Phylogenetic analysis showed that CtCVNH was grouped together with the two CVNHs from C. richardii. |
| 资助项目 | Chinese Academy of Sciences[0729281F02] ; National Natural Science Foundation of China[30771763] ; National Natural Science Foundation of China[30170101] ; Natural Science Foundation of Hubei Province, China[0631061H01] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000269677200002 |
| 出版者 | AMER FERN SOC INC |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/1044]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Su, Yingjuan |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 2.Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Xiaoqiong,Yang, Yongxia,Su, Yingjuan,et al. Molecular Cloning and Sequence Analysis of Cyanovirin-N Homology Gene in Ceratopteris thalictroides[J]. AMERICAN FERN JOURNAL,2009,99(2):78-92. |
| APA | Qi, Xiaoqiong,Yang, Yongxia,Su, Yingjuan,&Wang, Ting.(2009).Molecular Cloning and Sequence Analysis of Cyanovirin-N Homology Gene in Ceratopteris thalictroides.AMERICAN FERN JOURNAL,99(2),78-92. |
| MLA | Qi, Xiaoqiong,et al."Molecular Cloning and Sequence Analysis of Cyanovirin-N Homology Gene in Ceratopteris thalictroides".AMERICAN FERN JOURNAL 99.2(2009):78-92. |
入库方式： OAI收割
来源：武汉植物园
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