BAC-end sequence-based SNPs and Bin mapping for rapid integration of physical and genetic maps in apple
文献类型：期刊论文
| 作者 | Han, Yuepeng4,5; Chagne, David2; Gasic, Ksenija4; Rikkerink, Erik H. A.3; Beever, Jonathan E.1; Gardiner, Susan E.2; Korban, Schuyler S.4 |
| 刊名 | GENOMICS
 |
| 出版日期 | 2009-03-01 |
| 卷号 | 93期号:3页码:282-288 |
| 关键词 | Apple Single nucleotide polymorphisms Physical map Genetic map Bin mapping |
| ISSN号 | 0888-7543 |
| DOI | 10.1016/j.ygeno.2008.11.005 |
| 英文摘要 | A genome-wide BAC physical map of the apple, Malus x domestica Borkh., has been recently developed. Here, we report on integrating the physical and genetic maps of the apple using a SNP-based approach in conjunction with bin mapping. Briefly, BAC clones located at ends of BAC contigs were selected, and sequenced at both ends. The BAC end sequences (BESs) were used to identify candidate SNPs. Subsequently, these candidate SNPs were genetically mapped using a bin mapping strategy for the purpose of mapping the physical onto the genetic map. Using this approach, 52 (23%) out of 228 BESs tested were successfully exploited to develop SNPs. These SNPs anchored 51 contigs, spanning similar to 37 Mb in cumulative physical length, onto 14 linkage groups. The reliability of the integration of the physical and genetic maps using this SNP-based strategy is described, and the results confirm the feasibility of this approach to construct an integrated physical and genetic maps for apple. (C) 2008 Elsevier Inc. All rights reserved. |
| 资助项目 | USDA Cooperative State Research, Education and Extension Service - National Research Initiative-Plant Genome Program[2005-35300-15538] ; Illinois Council for Food and Agricultural Research[IDA CF 06FS-0303] ; New Zealand Foundation for Research Science and Technology (FRST)[C06XO207] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000263901000012 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/1054]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Korban, Schuyler S. |
| 作者单位 | 1.Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA 2.Palmerston N Ctr, Palmerston North 4442, New Zealand 3.Mt Albert Res Ctr, Auckland 1142, New Zealand 4.Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA 5.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Yuepeng,Chagne, David,Gasic, Ksenija,et al. BAC-end sequence-based SNPs and Bin mapping for rapid integration of physical and genetic maps in apple[J]. GENOMICS,2009,93(3):282-288. |
| APA | Han, Yuepeng.,Chagne, David.,Gasic, Ksenija.,Rikkerink, Erik H. A..,Beever, Jonathan E..,...&Korban, Schuyler S..(2009).BAC-end sequence-based SNPs and Bin mapping for rapid integration of physical and genetic maps in apple.GENOMICS,93(3),282-288. |
| MLA | Han, Yuepeng,et al."BAC-end sequence-based SNPs and Bin mapping for rapid integration of physical and genetic maps in apple".GENOMICS 93.3(2009):282-288. |
入库方式： OAI收割
来源：武汉植物园
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