Microsatellite-based molecular diversity of bread wheat germplasm and association mapping of wheat resistance to the Russian wheat aphid
文献类型：期刊论文
| 作者 | Peng, J. H.2,3; Bai, Y.1; Haley, S. D.3; Lapitan, N. L. V.3 |
| 刊名 | GENETICA
 |
| 出版日期 | 2009 |
| 卷号 | 135期号:1页码:95-122 |
| ISSN号 | 0016-6707 |
| 关键词 | Wheat germplasm Russian wheat aphid resistance Microsatellite Molecular diversity Genetic variation Association mapping |
| DOI | 10.1007/s10709-008-9262-x |
| 英文摘要 | Genetic diversity of a set of 71 wheat accessions, including 53 biotype 2 Russian wheat aphid (RWA2)-resistant landraces and 18 RWA2 susceptible accessions, was assessed by examining molecular variation at multiple microsatellite (SSR) loci. Fifty-one wheat SSR primer pairs were used, 81 SSR loci were determined, and 545 SSR alleles were detected. These SSR loci covered all the three genomes, 21 chromosomes, and at least 41 of the 42 chromosome arms. Diversity values averaged over SSR loci were high with mean number of SSR alleles/locus = 6.7, mean Shannon's index (H) = 1.291, and mean Nei's gene diversity (He) = 0.609. The three wheat genomes ranked as A > D > B and the homoeologous groups ranked as 7 > 3 > 1 > 2 > 6 > 5 > 4 based on the number of alleles per locus. Xgwm136 on chromosome arm 1AS is the most polymorphic SSR locus with the largest number of observed and effective alleles and the highest H and He. Among all 2485 pairs of wheat accessions, genetic distance (GD) ranged from 0.054 to 1.933 and averaged 0.9832. A dendrogram based on GD matrix showed that all the wheat accessions could be grouped into distinct clusters. Most of the susceptible cultivars (13/18) were clustered into groups that contains all or mostly susceptible accessions. Most of the U.S. cultivars belong to a group that is distinguishable from all the different RWA2 resistant groups. Diversity analysis was also conducted separately for subgroups containing 53 RWA2-resistant accessions and 18 RWA2-susceptible accessions. Association mapping revealed 28 SSR loci significantly associated with leaf chlorosis, and 8 with leaf rolling. New chromosome regions associated with RWA2 resistance were detected, and indicated existence of new RWA resistance genes located on chromosomes of all other homoeologous groups in addition to the groups 1 and 7 in bread wheat. This information is helpful for development of mapping populations for RWA2 resistance genes from different phylogenetic groups, and for wise utilization of the RWA-resistant germplasm in wheat breeding programs. |
| 资助项目 | Chinese Academy of Sciences[KSCX2-YW-Z0722] ; Colorado Wheat Research Foundation ; U. S. Department of Agriculture[2001-52100-11293] ; USDA[2003-34205-13636] ; National Research Initiative of USDA's Cooperative State Research, Education and Extension Service, CAP[2006-55606-16629] ; Hatch Funds[644] |
| WOS研究方向 | Genetics & Heredity |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000261176300011 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/1069]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Peng, J. H. |
| 作者单位 | 1.Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA 2.Chinese Acad Sci, Wuhan Bot Garden Inst, Wuhan 430074, Hubei, Peoples R China 3.Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA |
| 推荐引用方式 GB/T 7714 | Peng, J. H.,Bai, Y.,Haley, S. D.,et al. Microsatellite-based molecular diversity of bread wheat germplasm and association mapping of wheat resistance to the Russian wheat aphid[J]. GENETICA,2009,135(1):95-122. |
| APA | Peng, J. H.,Bai, Y.,Haley, S. D.,&Lapitan, N. L. V..(2009).Microsatellite-based molecular diversity of bread wheat germplasm and association mapping of wheat resistance to the Russian wheat aphid.GENETICA,135(1),95-122. |
| MLA | Peng, J. H.,et al."Microsatellite-based molecular diversity of bread wheat germplasm and association mapping of wheat resistance to the Russian wheat aphid".GENETICA 135.1(2009):95-122. |
入库方式： OAI收割
来源：武汉植物园
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