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Self-EcoTILLING to identify single-nucleotide mutations in multigene family

文献类型：期刊论文

作者Wang, Guang-Xi1; Imaizumi, Toshiyuki1; Li, Wei4; Saitoh, Hiromasa2; Terauchi, Ryohei2; Ohsako, Takanori3; Tominaga, Tohru1
刊名PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
出版日期2008-09-01
卷号92期号:1页码:24-29
关键词EcoTILLING multigene family polyploid plant Self-EcoTILLING single-nucleotide mutation
ISSN号0048-3575
DOI10.1016/j.pestbp.2008.05.001
英文摘要TILLING (Targeting Induced Local Lesions IN Genomes) is a low-cost, high-throughput reverse genetic technique that employs a mismatch-specific endonuclease CEL-1 to discover induced point mutations in the genes of interest. The use of the TILLING technique to survey natural variation in genes is called ECOTILLING. Here, we report a modified EcoTILLING method for the discovery of mutations in multigene family, which we coin "Self-EcoTILLING" by using an allotetraploid Monochoria vaginalis ALS multigene family as an example. The mutations could be detected by TILLING of PCR products resulting from the primers specific to both Als1 and Als3 without involving the experimental step of mixture of reference and query DNA. Either of the two co-amplified loci could serve as reference DNA to the other. We demonstrate with this example that Self-EcoTILLING is a fast, reliable and economical technique of detecting single-nucleotide mutations in polyploid plants containing multigene family. (C) 2008 Elsevier Inc. All rights reserved.
WOS研究方向Biochemistry & Molecular Biology ; Entomology ; Physiology
语种英语
WOS记录号WOS:000258560900005
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
源URL[http://202.127.146.157/handle/2RYDP1HH/1086]  
专题中国科学院武汉植物园
通讯作者Wang, Guang-Xi
作者单位1.Kyoto Univ, Grad Sch Agr, Weed Sci Lab, Kyoto 6068502, Japan
2.Iwate Biotechnol Res Ctr, Kitakami, Iwate 0240003, Japan
3.Kyoto Prefectural Univ, Grad Sch Agr, Lab Agroecol, Kyoto 6190244, Japan
4.Chinese Acad Sci, Wuhan Bot Garden, Hubei 430074, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Guang-Xi,Imaizumi, Toshiyuki,Li, Wei,et al. Self-EcoTILLING to identify single-nucleotide mutations in multigene family[J]. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,2008,92(1):24-29.
APA Wang, Guang-Xi.,Imaizumi, Toshiyuki.,Li, Wei.,Saitoh, Hiromasa.,Terauchi, Ryohei.,...&Tominaga, Tohru.(2008).Self-EcoTILLING to identify single-nucleotide mutations in multigene family.PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,92(1),24-29.
MLA Wang, Guang-Xi,et al."Self-EcoTILLING to identify single-nucleotide mutations in multigene family".PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 92.1(2008):24-29.

入库方式： OAI收割

来源：武汉植物园

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