Determination of atractylenolide II in rat plasma by reversed-phase high-performance liquid chromatography
文献类型：期刊论文
| 作者 | Ge, Jian; Wang, You-wei; Lu, Xiao-cong; Sun, Xiao-hong; Gong, Fu-jun |
| 刊名 | BIOMEDICAL CHROMATOGRAPHY
 |
| 出版日期 | 2007-03-01 |
| 卷号 | 21期号:3页码:299-303 |
| 关键词 | atractylenolide II RP-HPLC pharmacokinetics |
| ISSN号 | 0269-3879 |
| DOI | 10.1002/bmc.756 |
| 英文摘要 | A method for quantitative determination of atractylenolide 11 in rat plasma using reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with UV spectrometry was established. From a variety of compounds and solvents tested, atractylenolide III was selected as the internal standard (IS) and ethyl acetate was found to be the best solvent for extracting atractylenolide 11 from plasma samples. RP-HPLC analysis of the extracts was performed on an analytical column (DIKMA ODS, 150 x 4.6 mm: i.d.. 5 mu m) equipped with a security guard pre-column system. There was good linearity over the range 0.05-5.0 mu g/mL (r > 0.99). The recoveries were more than 90.0% in plasma, and the intra- and inter-day coefficients of variation were less than 10.0% in all cases. The limit of detection (LOD) was 0.025 mu g/mL and the lower limit of quantification (LLOQ) was 0.05 mu g/mL. The RP-HPLC method was applied to quantitate atractylenolide 11 in rat plasma within 24 h in a pharmacokinetics study where experimental rats received a single dose of atractylenolide 11 (60 mg/kg). Copyright (c) 2007 John Wiley & Sons, Ltd. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000245165100013 |
| 出版者 | JOHN WILEY & SONS LTD |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/1140]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Wang, You-wei |
| 作者单位 | 1.Grad Univ, Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China 3.Wuhan Univ, Coll Pharm, Wuhan 430072, Peoples R China 4.Hubei Ctr Dis Control & Prevent, Wuhan 430079, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Jian,Wang, You-wei,Lu, Xiao-cong,et al. Determination of atractylenolide II in rat plasma by reversed-phase high-performance liquid chromatography[J]. BIOMEDICAL CHROMATOGRAPHY,2007,21(3):299-303. |
| APA | Ge, Jian,Wang, You-wei,Lu, Xiao-cong,Sun, Xiao-hong,&Gong, Fu-jun.(2007).Determination of atractylenolide II in rat plasma by reversed-phase high-performance liquid chromatography.BIOMEDICAL CHROMATOGRAPHY,21(3),299-303. |
| MLA | Ge, Jian,et al."Determination of atractylenolide II in rat plasma by reversed-phase high-performance liquid chromatography".BIOMEDICAL CHROMATOGRAPHY 21.3(2007):299-303. |
入库方式： OAI收割
来源：武汉植物园
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