Determination of oridonin in rat plasma by reverse-phase high-performance liquid chromatography
文献类型：期刊论文
| 作者 | Jian Ge; Wang You-Wei; Lu Xiao-Cong; Cao Ji-Yue |
| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2007-01-17 |
| 卷号 | 43期号:2页码:793-797 |
| 关键词 | oridonin RP-HPLC pharmacokinetics |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2006.08.016 |
| 英文摘要 | A method for quantitative determination of oridonin in rat plasma using reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with UV spectrometry was established and the method was applied to a pharmacokinetics study of oridonin in rats. From a variety of compounds and solvents tested, ticolpidine was selected as the internal standard (IS) and ethyl acetate was found to be the best solvent for extracting oridonin from plasma samples. RP-HPLC analysis of the extracts was performed on an analytical column (DIKMA ODS, 200 mm x 4.6 mm; W., 5 mu m) equipped with a security guard pre-column system. There was a good linearity over the range 0.05-8.0 mu g/mL (r > 0.99). The recoveries were about 95.0% in plasma, and the intra- and inter-day coefficients of variation were less than 9.0% in all cases. The limit of detection (LOD) was 0.025 mu g/mL and the lower limit of quantification (LLOQ) was 0.05 mu g/mL. The RP-HPLC method was readily applied to quantitate oridonin in rat plasma within 24 h in a pharmacokinetics study where experimental rats received a single dose of oridonin (12.5 mg/kg) and the result was presented. (c) 2006 Elsevier B.V. All rights reserved. |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000243802700059 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/1144]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Wang You-Wei |
| 作者单位 | 1.Huazhong Agr Univ, Coll Vet Med, Wuhan 430070, Peoples R China 2.Wuhan Univ, Coll Pharm, Wuhan 430072, Peoples R China 3.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China 4.Hubei Ctr Dis Control & Prevent, Wuhan 430079, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jian Ge,Wang You-Wei,Lu Xiao-Cong,et al. Determination of oridonin in rat plasma by reverse-phase high-performance liquid chromatography[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2007,43(2):793-797. |
| APA | Jian Ge,Wang You-Wei,Lu Xiao-Cong,&Cao Ji-Yue.(2007).Determination of oridonin in rat plasma by reverse-phase high-performance liquid chromatography.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,43(2),793-797. |
| MLA | Jian Ge,et al."Determination of oridonin in rat plasma by reverse-phase high-performance liquid chromatography".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 43.2(2007):793-797. |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。