A two-stage process with temperature-shift for enhanced anthocyanin production in strawberry cell suspension cultures
文献类型：期刊论文
| 作者 | Zhang, W; Furusaki, S; Franco, C |
| 刊名 | science in china series b-chemistry
 |
| 出版日期 | 1999-08-01 |
| 卷号 | 42期号:4页码:345-350 |
| 关键词 | strawberry cell suspension cultures anthocyanin temperature two-stage culture temperature-shift |
| 英文摘要 | a two-stage process with temperature-shift has been developed to enhance the anthocyanin yield in suspension cultures of strawberry cells. the effect of the temperature-shift interval and the shift-time point was investigated for the optimization of this strategy. in this process, strawberry cells were grown at 30 degrees c (the optimum temperature for cell growth) for a certain period as the first stage, with the temperature shifted to a lower temperature for the second stage. in response to the temperature shift-down, anthocyanin synthesis was stimulated and a higher content could be achieved than that at both boundary temperatures but cell growth was suppressed. when the lower boundary temperature was decreased, cell growth was lowered and a delayed, sustained maximum anthocyanin content was achieved. anthocyanin synthesis was strongly influenced by the shift-time point but cell growth was not. consequently, the maximum anthocyanin content of 2.7 mg.g-fresh cell(-1) was obtained on day 9 by a temperature-shift from 30 degrees c, after 3-d culture, to 15 degrees c. the highest anthocyanin yield of 318 mg.l-1 on day 12 was achieved when the temperature was shifted from 30 degrees c, after 5-d culture, to 20 degrees c. for a global optimization of both the yield and productivity, the optimum anthocyanin yield and productivity of 272 mg.l-1 and 30.2 mg.l-1.d(-1) on day 9 were achieved by a two-stage culture with a temperature-shift from 30 degrees c after 3 d to 20 degrees c. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | catharanthus-roseus ; growth |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000082227600002 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/87339]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Flinders Univ S Australia, Sch Med, Bedford Pk, SA 5042, Australia 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biochem Engn, Dalian 116012, Peoples R China 3.Univ Tokyo, Grad Sch Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 113, Japan |
| 推荐引用方式 GB/T 7714 | Zhang, W,Furusaki, S,Franco, C. A two-stage process with temperature-shift for enhanced anthocyanin production in strawberry cell suspension cultures[J]. science in china series b-chemistry,1999,42(4):345-350. |
| APA | Zhang, W,Furusaki, S,&Franco, C.(1999).A two-stage process with temperature-shift for enhanced anthocyanin production in strawberry cell suspension cultures.science in china series b-chemistry,42(4),345-350. |
| MLA | Zhang, W,et al."A two-stage process with temperature-shift for enhanced anthocyanin production in strawberry cell suspension cultures".science in china series b-chemistry 42.4(1999):345-350. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。