Comparative study on alkaloids and their anti-proliferative activities from three Zanthoxylum species
文献类型：期刊论文
| 作者 | Tian, Yongqiang1,3; Zhang, Chunyun2,3; Guo, Mingquan2,3 |
| 刊名 | BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
 |
| 出版日期 | 2017-09-12 |
| 卷号 | 17页码:16 |
| 关键词 | Zanthoxylum alkaloids LC-UV-ESI-MS/MS Anti-proliferative activity Fingerprinting analysis |
| ISSN号 | 1472-6882 |
| DOI | 10.1186/s12906-017-1966-y |
| 英文摘要 | Background: Alkaloids have been considered as the most promising bioactive ingredients in plant species from the genus Zanthoxylum. This study reports on the compositions and contents of the Zanthoxylum alkaloids (ZAs) from three Zanthoxylum species, and their potential anti-proliferation activities. Methods: An HPLC-UV/ESI-MS/MS method was established and employed to analyze the alkaloids in different Zanthoxylum extracts. The common and unique peaks and their relative contents were summarized and compared to evaluate the similarity and dissimilarity of the three Zanthoxylum species. Meanwhile, inhibitory activity tests to four carcinoma cell lines, i.e., stomach tumor cells (SGC-7901), cervical tumor cells (Hela), colon tumor cells (HT-29) and Hepatic tumor cells (Hep G2), were carried out in vitro to evaluate the bioactivities of the ZAs. Results: Seventy peaks were detected in the crude total alkaloid samples, and 58 of them were identified. As a result, 13 common peaks were found in the extracts of all the three Zanthoxylum species, while some unique peaks were also observed in specific species, with 17 peaks in Z. simulans, 15 peaks in Z. ailanthoides and 11 peaks in Z. chalybeum, respectively. The comparison of the composition and relative contents indicated that alkaloids of benzophenanthridine type commonly present in all the three Zanthoxylum species with high relative contents among the others, which are 60.52% in Z. ailanthoides, 30.52% in Z. simulans and 13.84% in Z. chalybeum, respectively. In terms of activity test, Most of the crude alkaloids extracts showed remarkable inhibitory activities against various tumor cells, and the inhibitory rates ranged from 60.71 to 93. 63% at a concentration of 200 mu g/mL. However, SGC-7901 cells seemed to be more sensitive to the ZAs than the other three cancer cells. Conclusion: The alkaloid profiles detected in this work revealed significant differences in both structures and contents among Zanthoxylum species. The inhibitory rates for different cancer cells in this study indicated that the potential anti-cancer activity should be attributed to quaternary alkaloids in these three species, which will provide great guidance for further exploring this traditional medicinal resource as new healthcare products. |
| 资助项目 | Natural Science Foundation of China[81673580] ; Natural Science Foundation of China[31601800] ; Natural Science Foundation of Hubei Province of China[2016CFB285] ; Chinese Academy of Sciences[29Y429291a0129] ; Sino-Africa Joint Research Project[SAJC20160233] |
| WOS研究方向 | Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000410314700001 |
| 出版者 | BIOMED CENTRAL LTD |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/1309]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Guo, Mingquan |
| 作者单位 | 1.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Sinoafrica Joint Res Ctr, Wuhan 430074, Hubei, Peoples R China 3.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, Yongqiang,Zhang, Chunyun,Guo, Mingquan. Comparative study on alkaloids and their anti-proliferative activities from three Zanthoxylum species[J]. BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,2017,17:16. |
| APA | Tian, Yongqiang,Zhang, Chunyun,&Guo, Mingquan.(2017).Comparative study on alkaloids and their anti-proliferative activities from three Zanthoxylum species.BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,17,16. |
| MLA | Tian, Yongqiang,et al."Comparative study on alkaloids and their anti-proliferative activities from three Zanthoxylum species".BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE 17(2017):16. |
入库方式： OAI收割
来源：武汉植物园
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