Isolation and characterization of an endoparasite from the culture of oleaginous microalga Graesiella sp WBG-1
文献类型：期刊论文
| 作者 | Ding, Yi; Peng, Xinan; Wang, Zhongjie; Wen, Xiaobin; Geng, Yahong; Li, Yeguang |
| 刊名 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
 |
| 出版日期 | 2017-09-01 |
| 卷号 | 26页码:371-379 |
| ISSN号 | 2211-9264 |
| 关键词 | Oleaginous microalgae Endoparasite Amoeboaphelidium protococcarum Ultrastructure Host specificity |
| DOI | 10.1016/j.algal.2017.08.003 |
| 英文摘要 | Oleaginous microalgae are an important potential feedstock for biodiesel production. However, a problem in mass cultivation of microalgae is the frequent occurrence of infection by algal parasites. In the present study, an endoparasite WZ01 was isolated from an open raceway pond of oleaginous Graesiella sp. WBG-1, and caused epidemics resulting in algal population collapse. According to 18S rDNA-based phylogenetic analyses, morphology, ultrastructure and life cycle, strain WZ01 was identified as a member of Aphelidea, Amoeboaphelidium protococcarum, although it had some minor differences from A. protococcarum X-5 and FD95. Examination via transmission electron microscopy demonstrated that zoospores of the parasite WZ01 were amoeboid, which can produce two types of pseudopodia (multiple filopodia and short anterior lamellipodium), but the pseudocilium was not observed. Interestingly, amoeboid zoospores contained numerous dense-body vesicles, which have not been previously described. Moreover, our results revealed the presence of a microtubule inside the penetration tube, indicating that the contents of the cyst were injected into the host not only by vacuole pressure but also by using a microtubule-mediated mechanism. In addition, of the 42 tested algal species, only cultures of coccoid green algae Chlorococcum sp. A213 and Chlorococcum sp. GP1 rapidly and intensively developed infections by parasite WZ01. This is the first report on endoparasitic infection in oleaginous coccoid green algae. Our results will enhance understanding of parasite-host relationships, which will be beneficial in developing strategies for infection control. |
| 资助项目 | China Petrochemical Corporation[210080] ; China Petrochemical Corporation[215104] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000411731800041 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/1311]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Li, Yeguang |
| 作者单位 | Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Yi,Peng, Xinan,Wang, Zhongjie,et al. Isolation and characterization of an endoparasite from the culture of oleaginous microalga Graesiella sp WBG-1[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2017,26:371-379. |
| APA | Ding, Yi,Peng, Xinan,Wang, Zhongjie,Wen, Xiaobin,Geng, Yahong,&Li, Yeguang.(2017).Isolation and characterization of an endoparasite from the culture of oleaginous microalga Graesiella sp WBG-1.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,26,371-379. |
| MLA | Ding, Yi,et al."Isolation and characterization of an endoparasite from the culture of oleaginous microalga Graesiella sp WBG-1".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 26(2017):371-379. |
入库方式： OAI收割
来源：武汉植物园
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