Investigation of changes in endocannabinoids and N-acylethanolamides in biofluids, and their correlations with female infertility
文献类型：期刊论文
| 作者 | Xiao, HM; Ding, J; Luo, XT; Yao, YR; Guo, MQ |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2017 |
| 卷号 | 1509页码:16-25 |
| 英文摘要 | Female infertility is a worldwide medical problem, and the scarcity of infertility biomarkers has hindered the ability to launch preventive and therapeutic measures in a timely manner. Intriguingly, alterations in endocannabinoids (eCBs) and N-acylethanolamides (NAEs) have been observed in the biofluids of infertile females. Therefore, a hypothesis of using eCB and NAEs in biofluids as infertility biomarkers was proposed by several researchers; however, little evidence exists to verify the hypothesis. To investigate their correlations with female infertility, we developed a magnetic liquid microextraction-chemical derivatization (MLME-CD) method coupled with liquid chromatography-tandem mass spectrometry for the quantification of eCBs and NAEs in biofluids. The target compounds were first purified with magnetic toluene as sorbents, and then labeled with 4-(N,N-dimethyamino)benzoyl chloride (4-DMABC). The MLME-CD method offered several advantages, including reliable quantification results by preventing the isomerizadon of eCB, high throughput by requiring 20 min for sample preparation, and good sensitivity with limits of detection at 3.0-54.3 fmol. The intra-day and inter-day relative standard deviations were below 14.5%, and the recoveries were 87.4%-117.9%. Concentrations of eCBs and NAEs in the serum of 49 infertile women and 53 fertile women (controls), and in the ovarian follicular fluid of 21 infertile women and 20 controls were then quantified. Using unpaired t test analysis indicated significant differences in AEA and PEA in serum, and OEA in follicular fluid between infertile women and healthy controls, and the areas under the curve were in the range of 0.605-0.707. (C) 2017 The Authors. Published by Elsevier B.V. |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/1329]  |
| 专题 | 中国科学院武汉植物园 |
| 推荐引用方式 GB/T 7714 | Xiao, HM,Ding, J,Luo, XT,et al. Investigation of changes in endocannabinoids and N-acylethanolamides in biofluids, and their correlations with female infertility[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1509:16-25. |
| APA | Xiao, HM,Ding, J,Luo, XT,Yao, YR,&Guo, MQ.(2017).Investigation of changes in endocannabinoids and N-acylethanolamides in biofluids, and their correlations with female infertility.JOURNAL OF CHROMATOGRAPHY A,1509,16-25. |
| MLA | Xiao, HM,et al."Investigation of changes in endocannabinoids and N-acylethanolamides in biofluids, and their correlations with female infertility".JOURNAL OF CHROMATOGRAPHY A 1509(2017):16-25. |
入库方式： OAI收割
来源：武汉植物园
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