An efficient method for transgenic callus induction from Vitis amurensis petiole
文献类型：期刊论文
| 作者 | Zhao, Tingting1,2; Wang, Zemin1; Su, Lingye2,3; Sun, Xiaoming1,3; Cheng, Jun3; Zhang, Langlang1,2; Karungo, Sospeter Karanja1,2; Han, Yuepeng1; Li, Shaohua1,3; Xin, Haiping1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2017-06-22 |
| 卷号 | 12期号:6页码:13 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0179730 |
| 英文摘要 | Transformation is the main platform for genetic improvement and gene function studies in plants. However, the established somatic embryo transformation system for grapevines is time-consuming and has low efficiency, which limits its utilization in functional genomics research. Vitis amurensis is a wild Vitis species with remarkable cold tolerance. The lack of an efficient genetic transformation system for it has significantly hindered the functional identification of cold stress related genes in the species. Herein, an efficient method was established to produce transformed calli of V. amurensis. Segments of petioles from micro-propagated plantlets of V. amurensis exhibited better capacity to differentiate calli than leaf-discs and stem segments, and thus was chosen as target tissue for Agrobacterium-mediated transformation. Both neomycin phosphotransferase II (NPTII) and enhanced green fluorescent protein (eGFP) genes were used for simultaneous selection of transgenic calli based on kanamycin resistance and eGFP fluorescence. Several parameters affecting the transformation efficiency were optimized including the concentration of kanamycin, Agrobacterium stains, bacterial densities, infection treatments and co-cultivation time. The transgenic callus lines were verified by checking the integration of NPTII gene into calli genomes, the expression of eGFP gene and the fluorescence of eGFP. Up to 20% of the petiole segments produced transformed calli after 2 months of cultivation. This efficient transformation system will facilitate the functional analysis of agronomic characteristics and related genes not only in V. amurensis but also in other grapevine species. |
| 资助项目 | National Natural Science Foundation of China[31471857] ; National Natural Science Foundation of China[31672132] ; Youth Innovation Promotion Association of CAS[2015281] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000404135800057 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/1353]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Xin, Haiping |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, CAS Key Lab Plant Resources, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Tingting,Wang, Zemin,Su, Lingye,et al. An efficient method for transgenic callus induction from Vitis amurensis petiole[J]. PLOS ONE,2017,12(6):13. |
| APA | Zhao, Tingting.,Wang, Zemin.,Su, Lingye.,Sun, Xiaoming.,Cheng, Jun.,...&Xin, Haiping.(2017).An efficient method for transgenic callus induction from Vitis amurensis petiole.PLOS ONE,12(6),13. |
| MLA | Zhao, Tingting,et al."An efficient method for transgenic callus induction from Vitis amurensis petiole".PLOS ONE 12.6(2017):13. |
入库方式： OAI收割
来源：武汉植物园
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