Discovery of Several Novel Targets that Enhance beta-Carotene Production in Saccharomyces cerevisiae
文献类型：期刊论文
| 作者 | Li, Jia1; Shen, Jia1; Sun, Zhiqiang1; Li, Jing1; Li, Changfu1; Li, Xiaohua1,2; Zhang, Yansheng1 |
| 刊名 | FRONTIERS IN MICROBIOLOGY
 |
| 出版日期 | 2017-06-15 |
| 卷号 | 8页码:8 |
| 关键词 | beta-carotene biosynthesis Did2 colony-color screening novel amplifying targets Saccharomyces cerevisiae |
| ISSN号 | 1664-302X |
| DOI | 10.3389/fmicb.2017.01116 |
| 英文摘要 | beta-Carotene is the precursor of vitamin A, and also exhibits multiple pharmaceutical functions by itself. In comparison to chemical synthesis, the production of beta-carotene in microbes by metabolic engineering strategy is relatively inexpensive. Identifying genes enhancing beta-carotene production in microbes is important for engineering a strain of producing higher yields of beta-carotene. Most of previous efforts in identifying the gene targets have focused on the isoprenoid pathway where the beta-carotene biosynthesis belongs. However, due to the complex interactions between metabolic fluxes, seemingly irrelevant genes that are outside the isoprenoid pathway might also affect beta-carotene biosynthesis. To this end, here we provided an example that several novel gene targets, which are outside the isoprenoid pathway, have improving effects on beta-carotene synthesis in yeast cells, when they were over-expressed. Among these targets, the class E protein of the vacuolar protein-sorting pathway (Did2) led to the highest improvement in beta-carotene yields, which was 2.1-fold to that of the corresponding control. This improvement was further explained by the observation that the overexpression of the DID2 gene generally boosted the transcriptions of beta-carotene pathway genes. The mechanism by which the other targets improve the production of beta-carotene is discussed. |
| 资助项目 | Grant for One Hundred Talents Program of the Chinese Academy of Sciences, China[Y129441R01] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000403308700001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/1354]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhang, Yansheng |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jia,Shen, Jia,Sun, Zhiqiang,et al. Discovery of Several Novel Targets that Enhance beta-Carotene Production in Saccharomyces cerevisiae[J]. FRONTIERS IN MICROBIOLOGY,2017,8:8. |
| APA | Li, Jia.,Shen, Jia.,Sun, Zhiqiang.,Li, Jing.,Li, Changfu.,...&Zhang, Yansheng.(2017).Discovery of Several Novel Targets that Enhance beta-Carotene Production in Saccharomyces cerevisiae.FRONTIERS IN MICROBIOLOGY,8,8. |
| MLA | Li, Jia,et al."Discovery of Several Novel Targets that Enhance beta-Carotene Production in Saccharomyces cerevisiae".FRONTIERS IN MICROBIOLOGY 8(2017):8. |
入库方式： OAI收割
来源：武汉植物园
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