Molecular characterization of the C-glucosylation for puerarin biosynthesis in Pueraria lobata
文献类型：期刊论文
| 作者 | Wang, Xin1; Li, Changfu1; Zhou, Chen1,2; Li, Jia1; Zhang, Yansheng1,3 |
| 刊名 | PLANT JOURNAL
 |
| 出版日期 | 2017-05-01 |
| 卷号 | 90期号:3页码:535-546 |
| 关键词 | Pueraria lobata transcriptome C-glucosyltransferase soybean hairy roots isoflavone |
| ISSN号 | 0960-7412 |
| DOI | 10.1111/tpj.13510 |
| 英文摘要 | C-glycosyltransferases (CGTs) are important enzymes that are responsible for the synthesis of the C-glycosides of flavonoids and isoflavonoids. Flavonoid CGTs have been molecularly characterized from several plant species; however, to date, no gene encoding an isoflavonoid CGT has been reported from any plant species. A significant example of an isoflavonoid C-glycoside is puerarin, a compound that contributes to the major medicinal effects of Pueraria lobata. Little is known about the C-glucosylation that occurs during puerarin biosynthesis. One possible route for puerarin synthesis is via the C-glucosylation of daidzein. This study describes the molecular cloning and functional characterization of a novel glucosyltransferase (PlUGT43) from P.lobata. Biochemical analyses revealed that PlUGT43 possesses an activity for the C-glucosylation of daidzein to puerarin; it shows activity with the isoflavones daidzein and genistein, but displays no activity towards other potential acceptors, including flavonoids. To validate the invivo function of PlUGT43, the PlUGT43 gene was over-expressed in soybean hairy roots that naturally synthesize daidzein but that do not produce puerarin. The expression of PlUGT43 led to the production of puerarin in the transgenic soybean hairy roots, confirming a role for PlUGT43 in puerarin biosynthesis. |
| 资助项目 | National Science Foundation of China[31170284] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000399732200008 |
| 出版者 | WILEY |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/1380]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhang, Yansheng |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan Bot Garden, Wuhan 430074, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Sinoafrican Joint Res Ctr, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xin,Li, Changfu,Zhou, Chen,et al. Molecular characterization of the C-glucosylation for puerarin biosynthesis in Pueraria lobata[J]. PLANT JOURNAL,2017,90(3):535-546. |
| APA | Wang, Xin,Li, Changfu,Zhou, Chen,Li, Jia,&Zhang, Yansheng.(2017).Molecular characterization of the C-glucosylation for puerarin biosynthesis in Pueraria lobata.PLANT JOURNAL,90(3),535-546. |
| MLA | Wang, Xin,et al."Molecular characterization of the C-glucosylation for puerarin biosynthesis in Pueraria lobata".PLANT JOURNAL 90.3(2017):535-546. |
入库方式： OAI收割
来源：武汉植物园
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