Transcriptome and metabolome responses of Shewanella oneidensis MR-1 to methyl orange under microaerophilic and aerobic conditions
文献类型：期刊论文
| 作者 | Cao, Xinhua1,3; Qi, Yueling1,3; Xu, Chen1,3; Yang, Yuyi1; Wang, Jun1,2 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2017-04-01 |
| 卷号 | 101期号:8页码:3463-3472 |
| ISSN号 | 0175-7598 |
| 关键词 | Metabolome Transcriptome Azo dye Dissolved oxygen Biodegradation mechanism |
| DOI | 10.1007/s00253-016-8087-2 |
| 英文摘要 | Shewanella oneidensis MR-1 degrades various azo dyes under microaerophilic and anaerobic conditions, but this process is inhibited under aerobic conditions. The mechanisms underlying azo dye biodegradation and inhibition remain unknown. Therefore, we investigated metabolic and transcriptional changes in strain MR-1, which was cultured under different conditions, to elucidate these mechanisms. At the transcriptional level, genes involved in certain metabolic processes, particularly the tricarboxylic acid (TCA) cycle, amino acid biodegradation, and the electron transfer system, were significantly altered (M >= 2, p > 0.8 ) in the presence of methyl orange (MO). Moreover, a high concentration of dissolved oxygen heavily impacted the expression levels of genes involved in fatty acid biodegradation. Metabolome analysis revealed significant alteration (p < 0.05) in the concentrations of nine metabolites when strain MR-1 was cultured under aerobic conditions; the majority of these metabolites were closely associated with amino acid metabolism and DNA replication. Accordingly, we propose a possible pathway for MO biodegradation and discuss the most likely causes of biodegradation inhibition due to dissolved oxygen. |
| 资助项目 | National Natural Science Foundation of China[31400113] ; Funding Project of Sino-Africa Joint Research Center, Chinese Academy of Sciences[Y623321K01] ; Hundred Talents Program of the Chinese Academy of Science[Y329671K01] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000399167800038 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/1394]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Yang, Yuyi; Wang, Jun |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Aquat Bot & Watershed Ecol, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Sinoafrica Joint Res Ctr, Wuhan 430074, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Xinhua,Qi, Yueling,Xu, Chen,et al. Transcriptome and metabolome responses of Shewanella oneidensis MR-1 to methyl orange under microaerophilic and aerobic conditions[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2017,101(8):3463-3472. |
| APA | Cao, Xinhua,Qi, Yueling,Xu, Chen,Yang, Yuyi,&Wang, Jun.(2017).Transcriptome and metabolome responses of Shewanella oneidensis MR-1 to methyl orange under microaerophilic and aerobic conditions.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,101(8),3463-3472. |
| MLA | Cao, Xinhua,et al."Transcriptome and metabolome responses of Shewanella oneidensis MR-1 to methyl orange under microaerophilic and aerobic conditions".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 101.8(2017):3463-3472. |
入库方式： OAI收割
来源：武汉植物园
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