Effect of flexible linker length on the activity of fusion protein 4-coumaroyl-CoA ligase:: stilbene synthase
文献类型：期刊论文
| 作者 | Guo, HL; Yang, YD; Xue, FY; Zhang, H; Huang, TR; Liu, WB; Liu, H; Zhang, FQ; Yang, MF; Liu, CM |
| 刊名 | MOLECULAR BIOSYSTEMS
 |
| 出版日期 | 2017 |
| 卷号 | 13期号:3页码:598-606 |
| 英文摘要 | In order to elucidate the effect of flexible linker length on the catalytic efficiency of fusion proteins, two short flexible peptide linkers of various lengths were fused between Arabidopsis thaliana 4-coumaroyl-CoA ligase (4CL) and Polygonum cuspidatum stilbene synthase (STS) to generate fusion proteins 4CL-(GSG)(n)-STS (n <= 5) and 4CL-(GGGGS)(n)-STS (n <= 4). The fusion proteins were expressed in both Escherichia coli and Saccharomyces cerevisiae, and their bioactivities were tested in vitro and in vivo using purified proteins and engineered strains, respectively. The catalytic efficiency of the fusions decreased gradually with the increase of GSG or GGGGS repeats. In both engineered S. cerevisiae and E. coli in vivo experiments, the capacity of resveratrol production decreased gradually with increasing linker length. In silico analysis showed that the prediction of homology models of fusion proteins was consistent with the in vitro and in vivo results. |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/1409]  |
| 专题 | 中国科学院武汉植物园 |
| 推荐引用方式 GB/T 7714 | Guo, HL,Yang, YD,Xue, FY,et al. Effect of flexible linker length on the activity of fusion protein 4-coumaroyl-CoA ligase:: stilbene synthase[J]. MOLECULAR BIOSYSTEMS,2017,13(3):598-606. |
| APA | Guo, HL.,Yang, YD.,Xue, FY.,Zhang, H.,Huang, TR.,...&Ma, LQ.(2017).Effect of flexible linker length on the activity of fusion protein 4-coumaroyl-CoA ligase:: stilbene synthase.MOLECULAR BIOSYSTEMS,13(3),598-606. |
| MLA | Guo, HL,et al."Effect of flexible linker length on the activity of fusion protein 4-coumaroyl-CoA ligase:: stilbene synthase".MOLECULAR BIOSYSTEMS 13.3(2017):598-606. |
入库方式： OAI收割
来源：武汉植物园
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