Functional characterization and expression of GASCL1 and GASCL2, two anther-specific chalcone synthase like enzymes from Gerbera hybrida
文献类型：期刊论文
| 作者 | Kontturi, Juha1; Osama, Raisa1; Deng, Xianbao1,4; Bashandy, Hany1,2; Albert, Victor A.3; Teeri, Teemu H.1 |
| 刊名 | PHYTOCHEMISTRY
 |
| 出版日期 | 2017-02-01 |
| 卷号 | 134页码:38-45 |
| 关键词 | Gerbera hybrida Asteraceae Type III polyketide synthase ASCL Sporopollenin |
| ISSN号 | 0031-9422 |
| DOI | 10.1016/j.phytochem.2016.11.002 |
| 英文摘要 | The chalcone synthase superfamily consists of type III polyketidesynthases (PKSs), enzymes responsible for producing plant secondary metabolites with various biological and pharmacological activities. Anther-specific chalcone synthase-like enzymes (ASCLs) represent an ancient group of type III PKSs involved in the biosynthesis of sporopollenin, the main component of the exine layer of moss spores and mature pollen grains of seed plants. In the latter, ASCL proteins are localized in the tapetal cells of the anther where they participate in sporopollenin biosynthesis and exine formation within the locule. It is thought that the enzymes responsible for sporopollenin biosynthesis are highly conserved, and thus far, each angiosperm species with a genome sequenced has possessed two ASCL genes, which in Arabidopsis thaliana are PKSA and PKSB. The Gerbera hybrida (gerbera) PKS protein family consists of three chalcone synthases (GCHS1, GCHS3 and GCHS4) and three 2-pyrone synthases (G2PS1, G2PS2 and G2PS3). In previous studies we have demonstrated the functions of chalcone synthases in flavonoid biosynthesis, and the involvement of 2-pyrone synthases in the biosynthesis of antimicrobial compounds found in gerbera. In this study we expanded the gerbera PKS-family by functionally characterizing two gerbera ASCL proteins. In vitro enzymatic studies using purified recombinant proteins showed that both GASCL1 and GASCL2 were able to use medium and long-chain acyl-CoA starters and perform two to three condensation reactions of malonyl-CoA to produce tri- and tetraketide 2-pyrones, usually referred to as alpha-pyrones in sporopollenin literature. Both GASCL1 and GASCL2 genes were expressed only floral organs, with most expression observed in anthers. In the anthers, transcripts of both genes showed strict tapetum-specific localization. (C) 2016 Elsevier Ltd. All rights reserved. |
| 资助项目 | Academy of Finland[139513] ; Doctoral Programme in Plant Sciences (DPPS) at the University of Helsinki |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000393928600003 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/1419]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Teeri, Teemu H. |
| 作者单位 | 1.Univ Helsinki, Viikki Plant Sci Ctr, Dept Agr Sci, POB 27, FIN-00014 Helsinki, Finland 2.Cairo Univ, Dept Genet, 13 Gamaa St, Giza 12619, Egypt 3.Univ Buffalo, Dept Biol Sci, Buffalo, NY USA 4.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Kontturi, Juha,Osama, Raisa,Deng, Xianbao,et al. Functional characterization and expression of GASCL1 and GASCL2, two anther-specific chalcone synthase like enzymes from Gerbera hybrida[J]. PHYTOCHEMISTRY,2017,134:38-45. |
| APA | Kontturi, Juha,Osama, Raisa,Deng, Xianbao,Bashandy, Hany,Albert, Victor A.,&Teeri, Teemu H..(2017).Functional characterization and expression of GASCL1 and GASCL2, two anther-specific chalcone synthase like enzymes from Gerbera hybrida.PHYTOCHEMISTRY,134,38-45. |
| MLA | Kontturi, Juha,et al."Functional characterization and expression of GASCL1 and GASCL2, two anther-specific chalcone synthase like enzymes from Gerbera hybrida".PHYTOCHEMISTRY 134(2017):38-45. |
入库方式： OAI收割
来源：武汉植物园
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