Digital Gene Expression Analysis Provides Insight into the Transcript Profile of the Genes Involved in Aporphine Alkaloid Biosynthesis in Lotus (Nelumbo nucifera)
文献类型：期刊论文
| 作者 | Yang, Mei1; Zhu, Lingping1,4; Li, Ling1,3; Li, Juanjuan1,3; Xu, Liming1; Feng, Ji2; Liu, Yanling1 |
| 刊名 | Frontiers in Plant Science
 |
| 出版日期 | 2017-01-31 |
| 卷号 | 8页码:19 |
| 关键词 | lotus aporphine alkaloids co-expression network biosynthesis putative genes |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2017.00080 |
| 英文摘要 | The predominant alkaloids in lotus leaves are aporphine alkaloids. These are the most important active components and have many pharmacological properties, but little is known about their biosynthesis. We used digital gene expression (DGE) technology to identify differentially-expressed genes (DEGs) between two lotus cultivars with different alkaloid contents at four leaf development stages. We also predicted potential genes involved in aporphine alkaloid biosynthesis by weighted gene co-expression network analysis (WGCNA). Approximately 335 billion nucleotides were generated; and 94% of which were aligned against the reference genome. Of 22 thousand expressed genes, 19,000 were differentially expressed between the two cultivars at the four stages. Gene Ontology (GO) enrichment analysis revealed that catalytic activity and oxidoreductase activity were enriched significantly in most pairwise comparisons. In Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, dozens of DEGs were assigned to the categories of biosynthesis of secondary metabolites, isoquinoline alkaloid biosynthesis, and flavonoid biosynthesis. The genes encoding norcoclaurine synthase (NCS), norcoclaurine 6-O-methyltransferase (6OMT), coclaurine N-methyltransferase (CNMT), N-methylcoclaurine 3'-hydroxylase (NMCH), and 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase (4'OMT) in the common pathways of benzylisoquinoline alkaloid biosynthesis and the ones encoding corytuberine synthase (CTS) in aporphine alkaloid biosynthetic pathway, which have been characterized in other plants, were identified in lotus. These genes had positive effects on alkaloid content, albeit with phenotypic lag. The WGCNA of DEGs revealed that one network module was associated with the dynamic change of alkaloid content. Eleven genes encoding proteins with methyltransferase, oxidoreductase and CYP450 activities were identified. These were surmised to be genes involved in aporphine alkaloid biosynthesis. This transcriptomic database provides new directions for future studies on clarifying the aporphine alkaloid pathway. |
| 资助项目 | National Natural Science Foundation of China[31272195] ; National Natural Science Foundation of China[31471899] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000392864200001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/1421]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Liu, Yanling |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Aquat Plant & Watershed Ecol, Wuhan, Peoples R China 2.Tobacco Res Inst Hubei Prov, Wuhan, Peoples R China 3.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 4.Univ Helsinki, Viikki Plant Sci Ctr, Dept Agr Sci, Helsinki, Finland |
| 推荐引用方式 GB/T 7714 | Yang, Mei,Zhu, Lingping,Li, Ling,et al. Digital Gene Expression Analysis Provides Insight into the Transcript Profile of the Genes Involved in Aporphine Alkaloid Biosynthesis in Lotus (Nelumbo nucifera)[J]. Frontiers in Plant Science,2017,8:19. |
| APA | Yang, Mei.,Zhu, Lingping.,Li, Ling.,Li, Juanjuan.,Xu, Liming.,...&Liu, Yanling.(2017).Digital Gene Expression Analysis Provides Insight into the Transcript Profile of the Genes Involved in Aporphine Alkaloid Biosynthesis in Lotus (Nelumbo nucifera).Frontiers in Plant Science,8,19. |
| MLA | Yang, Mei,et al."Digital Gene Expression Analysis Provides Insight into the Transcript Profile of the Genes Involved in Aporphine Alkaloid Biosynthesis in Lotus (Nelumbo nucifera)".Frontiers in Plant Science 8(2017):19. |
入库方式： OAI收割
来源：武汉植物园
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