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A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome highlights the challenges associated with draft genomes and gene prediction in plants
文献类型:期刊论文
作者 | Pilkington,Sarah M.3; Crowhurst,Ross3; Hilario,Elena3; Nardozza,Simona3; Fraser,Lena3; Peng,Yongyan3,4; Gunaseelan,Kularajathevan3; Simpson,Robert5; Tahir,Jibran5; Deroles,Simon C.5 |
刊名 | BMC Genomics |
出版日期 | 2018-04-16 |
卷号 | 19期号:1 |
ISSN号 | 1471-2164 |
关键词 | Manual annotation Genome sequencing Actinidia chinensis |
DOI | 10.1186/s12864-018-4656-3 |
英文摘要 | AbstractBackgroundMost published genome sequences are drafts, and most are dominated by computational gene prediction. Draft genomes typically incorporate considerable sequence data that are not assigned to chromosomes, and predicted genes without quality confidence measures. The current Actinidia chinensis (kiwifruit) ‘Hongyang’ draft genome has 164?Mb of sequences unassigned to pseudo-chromosomes, and omissions have been identified in the gene models.ResultsA second genome of an A. chinensis (genotype Red5) was fully sequenced. This new sequence resulted in a 554.0?Mb assembly with all but 6?Mb assigned to pseudo-chromosomes. Pseudo-chromosomal comparisons showed a considerable number of translocation events have occurred following a whole genome duplication (WGD) event some consistent with centromeric Robertsonian-like translocations. RNA sequencing data from 12 tissues and ab initio analysis informed a genome-wide manual annotation, using the WebApollo tool. In total, 33,044 gene loci represented by 33,123 isoforms were identified, named and tagged for quality of evidential support. Of these 3114 (9.4%) were identical to a protein within ‘Hongyang’ The Kiwifruit Information Resource (KIR v2). Some proportion of the differences will be varietal polymorphisms. However, as most computationally predicted Red5 models required manual re-annotation this proportion is expected to be small. The quality of the new gene models was tested by fully sequencing 550 cloned ‘Hort16A’ cDNAs and comparing with the predicted protein models for Red5 and both the original ‘Hongyang’ assembly and the revised annotation from KIR v2. Only 48.9% and 63.5% of the cDNAs had a match with 90% identity or better to the original and revised ‘Hongyang’ annotation, respectively, compared with 90.9% to the Red5 models.ConclusionsOur study highlights the need to take a cautious approach to draft genomes and computationally predicted genes. Our use of the manual annotation tool WebApollo facilitated manual checking and correction of gene models enabling improvement of computational prediction. This utility was especially relevant for certain types of gene families such as the EXPANSIN like genes. Finally, this high quality gene set will supply the kiwifruit and general plant community with a new tool for genomics and other comparative analysis. |
语种 | 英语 |
出版者 | BioMed Central |
WOS记录号 | BMC:10.1186/S12864-018-4656-3 |
源URL | [http://202.127.146.157/handle/2RYDP1HH/1465] |
专题 | 中国科学院武汉植物园 |
通讯作者 | Schaffer,Robert J. |
作者单位 | 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. |
推荐引用方式 GB/T 7714 | Pilkington,Sarah M.,Crowhurst,Ross,Hilario,Elena,et al. A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome highlights the challenges associated with draft genomes and gene prediction in plants[J]. BMC Genomics,2018,19(1). |
APA | Pilkington,Sarah M..,Crowhurst,Ross.,Hilario,Elena.,Nardozza,Simona.,Fraser,Lena.,...&Schaffer,Robert J..(2018).A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome highlights the challenges associated with draft genomes and gene prediction in plants.BMC Genomics,19(1). |
MLA | Pilkington,Sarah M.,et al."A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome highlights the challenges associated with draft genomes and gene prediction in plants".BMC Genomics 19.1(2018). |
入库方式: OAI收割
来源:武汉植物园
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