Combined Metabonomic and Quantitative RT-PCR Analyses Revealed Metabolic Reprogramming Associated with Fusarium graminearum Resistance in Transgenic Arabidopsis thaliana
文献类型：期刊论文
| 作者 | Chen, Fangfang1,2,3; Liu, Caixiang5; Zhang, Jingtao5; Lei, Hehua5; Li, He-Ping2,3; Liao, Yu-Cai2,3; Tang, Huiru4,5,6 |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2018-01-04 |
| 卷号 | 8页码:14 |
| 关键词 | Arabidopsis thaliana Fusarium graminearum metabolic responses mycotoxins Chi-CWP2 NMR |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2017.02177 |
| 英文摘要 | Fusarium head blight disease resulting from Fusarium graminearum (FG) infection causes huge losses in global production of cereals and development of FG-resistant plants is urgently needed. To understand biochemistry mechanisms for FG resistance, here, we have systematically investigated the plant metabolomic phenotypes associated with FG resistance for transgenic Arabidopsis thaliana expressing a class-I chitinase (Chi), a Fusarium-specific recombinant antibody gene (CWP2) and fused Chi-CWP2. Plant disease indices, mycotoxin levels, metabonomic characteristics, and expression levels of several key genes were measured together with their correlations. We found that A. thaliana expressing Chi-CWP2 showed higher FG resistance with much lower disease indices and mycotoxin levels than the wild-type and the plants expressing Chi or CWP2 alone. The combined metabonomic and quantitative RT-PCR analyses revealed that such FG-resistance was closely associated with the promoted biosynthesis of secondary metabolites (phenylpropanoids, alkanoids) and organic osmolytes (proline, betaine, glucose, myo-inositol) together with enhanced TCA cycle and GABA shunt. These suggest that the concurrently enhanced biosyntheses of the shikimate-mediated secondary metabolites and organic osmolytes be an important strategy for A. thaliana to develop and improve FG resistance. These findings provide essential biochemical information related to FG resistance which is important for developing FG-resistant cereals. |
| 资助项目 | National Natural Science Foundation of China[31600240] ; National Natural Science Foundation of China[31200208] ; National Natural Science Foundation of China[21175149] ; National Natural Science Foundation of China[31272004] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000419330100001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/3372]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Liao, Yu-Cai; Tang, Huiru |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan Bot Garden, Wuhan, Hubei, Peoples R China 2.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan, Peoples R China 3.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan, Peoples R China 4.Fudan Univ, Sch Life Sci, Collaborat Innovat Ctr Genet & Dev, Shanghai Int Ctr Mol Phen,Metabon & Syst Biol Lab, Shanghai, Peoples R China 5.Chinese Acad Sci, Wuhan Inst Phys & Math, Natl Ctr Magnet Resonance Wuhan, CAS Key Lab Magnet Resonance Biol Syst,State Key, Wuhan, Hubei, Peoples R China 6.Fudan Univ, State Key Lab Genet Engn, Zhongshan Hosp, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Fangfang,Liu, Caixiang,Zhang, Jingtao,et al. Combined Metabonomic and Quantitative RT-PCR Analyses Revealed Metabolic Reprogramming Associated with Fusarium graminearum Resistance in Transgenic Arabidopsis thaliana[J]. FRONTIERS IN PLANT SCIENCE,2018,8:14. |
| APA | Chen, Fangfang.,Liu, Caixiang.,Zhang, Jingtao.,Lei, Hehua.,Li, He-Ping.,...&Tang, Huiru.(2018).Combined Metabonomic and Quantitative RT-PCR Analyses Revealed Metabolic Reprogramming Associated with Fusarium graminearum Resistance in Transgenic Arabidopsis thaliana.FRONTIERS IN PLANT SCIENCE,8,14. |
| MLA | Chen, Fangfang,et al."Combined Metabonomic and Quantitative RT-PCR Analyses Revealed Metabolic Reprogramming Associated with Fusarium graminearum Resistance in Transgenic Arabidopsis thaliana".FRONTIERS IN PLANT SCIENCE 8(2018):14. |
入库方式： OAI收割
来源：武汉植物园
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