Diversity of Growth Patterns Probed in Live Cyanobacterial Cells Using a Fluorescent Analog of a Peptidoglycan Precursor
文献类型：期刊论文
| 作者 | Zhang, Ju-Yuan1; Lin, Gui-Ming1; Xing, Wei-Yue1,2; Zhang, Cheng-Cai1 |
| 刊名 | FRONTIERS IN MICROBIOLOGY
 |
| 出版日期 | 2018-04-24 |
| 卷号 | 9页码:10 |
| 关键词 | cyanobacteria peptidoglycan growth pattern cell wall heterocyst HADA |
| ISSN号 | 1664-302X |
| DOI | 10.3389/fmicb.2018.00791 |
| 英文摘要 | Cyanobacteria were the first oxygenic photosynthetic organisms during evolution and were ancestors of plastids. Cyanobacterial cells exhibit an extraordinary diversity in their size and shape, and bacterial cell morphology largely depends on the synthesis and the dynamics of the peptidoglycan (PG) layer. Here, we used a fluorescence analog of the PG synthesis precursor D-Ala, 7-Hydroxycoumarin-amino-D-alanine (HADA), to probe the PG synthesis pattern in live cells of cyanobacteria with different morphology. They displayed diverse synthesis patterns, with some strains showing an intensive HADA incorporation at the septal region, whereas others gave an HADA signal distributed around the cells. Growth zones covering several cells at the tips of the filament were present in some filamentous strains such as in Arthrospira. In Anabaena PCC 7120, which is capable of differentiating heterocysts for N-2 fixation, PG synthesis followed the cell division cycle. In addition, an HADA incorporation was strongly activated from 12 to 15 h following the initiation of heterocyst development, indicating a thickening of the PG layer in heterocysts. The PG synthesis pattern is diverse in cyanobacteria and responds to developmental regulation. The use of fluorescent analogs may serve as a useful tool for understanding the mechanisms of cell growth and morphogenesis operating in these organisms. |
| 资助项目 | Key Research Program of Frontier Sciences of the Chinese Academy of Sciences[QYZDJ-SSW-SMC016] ; Recruitment Program of Global Experts of China[Y523011] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000430711800001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/3395]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhang, Cheng-Cai |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Ju-Yuan,Lin, Gui-Ming,Xing, Wei-Yue,et al. Diversity of Growth Patterns Probed in Live Cyanobacterial Cells Using a Fluorescent Analog of a Peptidoglycan Precursor[J]. FRONTIERS IN MICROBIOLOGY,2018,9:10. |
| APA | Zhang, Ju-Yuan,Lin, Gui-Ming,Xing, Wei-Yue,&Zhang, Cheng-Cai.(2018).Diversity of Growth Patterns Probed in Live Cyanobacterial Cells Using a Fluorescent Analog of a Peptidoglycan Precursor.FRONTIERS IN MICROBIOLOGY,9,10. |
| MLA | Zhang, Ju-Yuan,et al."Diversity of Growth Patterns Probed in Live Cyanobacterial Cells Using a Fluorescent Analog of a Peptidoglycan Precursor".FRONTIERS IN MICROBIOLOGY 9(2018):10. |
入库方式： OAI收割
来源：武汉植物园
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