Current advances in screening for bioactive components from medicinal plants by affinity ultrafiltration mass spectrometry
文献类型：期刊论文
| 作者 | Chen, Guilin1,2; Huang, Bill X.3; Guo, Mingquan1,2 |
| 刊名 | PHYTOCHEMICAL ANALYSIS
 |
| 出版日期 | 2018-07-01 |
| 卷号 | 29期号:4页码:375-386 |
| ISSN号 | 0958-0344 |
| 关键词 | active components affinity UF-LC MS biological targets high-throughput medicinal plants |
| DOI | 10.1002/pca.2769 |
| 英文摘要 | IntroductionMedicinal plants have played an important role in maintaining human health for thousands of years. However, the interactions between the active components in medicinal plants and some certain biological targets during a disease are still unclear in most cases. ObjectiveTo conduct the high-throughput screening for small active molecules that can interact with biological targets, which is of great theoretical significance and practical value. MethodologyThe ultrafiltration mass spectrometry (UF-LC/MS) is a powerful bio-analytical method by combining affinity ultrafiltration and liquid chromatography-mass spectrometry (LC/MS), which could rapidly screen and identify small active molecules that bind to biological targets of interest at the same time. Compared with other analytical methods, affinity UF-LC/MS has the characteristics of fast, sensitive and high throughput, and is especially suitable for the complicated extracts of medicinal plants. ResultsIn this review, the basic principle, characteristics and some most recent challenges in UF-LC/MS have been demonstrated. Meanwhile, the progress and applications of affinity UF-LC/MS in the discovery of the active components from natural medicinal plants and the interactions between small molecules and biological target proteins are also briefly summarised. In addition, the future directions for UF-LC/MS are also prospected. ConclusionAffinity UF-LC/MS is a powerful tool in studies on the interactions between small active molecules and biological protein targets, especially in the high-throughput screening of active components from the natural medicinal plants. The most recent development of UF-MS, and its applications in the identification of targeted bioactive components from medicinal plants have been reviewed. As a highly selective method for bio-affinity capture and high through-put screening, UF-MS is very useful not only for screening of bioactive molecules, but also for the evaluation of the quality, and efficacy of medicinal plants. |
| 资助项目 | Chinese Academy of Sciences[29Y429291a0129] ; Major Project for Special Technology Innovation of Hubei Province -- Sino-foreign Cooperation Project[2017AHB054] ; Natural Science Foundation of China[81673580] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000435641700006 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/3409]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Guo, Mingquan |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China 2.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Hubei, Peoples R China 3.NIAAA, Lab Mol Signaling, NIH, Bethesda, MD USA |
| 推荐引用方式 GB/T 7714 | Chen, Guilin,Huang, Bill X.,Guo, Mingquan. Current advances in screening for bioactive components from medicinal plants by affinity ultrafiltration mass spectrometry[J]. PHYTOCHEMICAL ANALYSIS,2018,29(4):375-386. |
| APA | Chen, Guilin,Huang, Bill X.,&Guo, Mingquan.(2018).Current advances in screening for bioactive components from medicinal plants by affinity ultrafiltration mass spectrometry.PHYTOCHEMICAL ANALYSIS,29(4),375-386. |
| MLA | Chen, Guilin,et al."Current advances in screening for bioactive components from medicinal plants by affinity ultrafiltration mass spectrometry".PHYTOCHEMICAL ANALYSIS 29.4(2018):375-386. |
入库方式： OAI收割
来源：武汉植物园
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