Overexpression of ethylene response factors VaERF080 and VaERF087 from Vitis amurensis enhances cold tolerance in Arabidopsis
文献类型：期刊论文
| 作者 | Sun, Xiaoming1,4; Zhu, Zhenfei1,3; Zhang, Langlang1; Fang, Linchuan1; Zhang, Jisen2; Wang, Qingfeng1; Li, Shaohua4; Liang, Zhenchang4; Xin, Haiping1 |
| 刊名 | SCIENTIA HORTICULTURAE
 |
| 出版日期 | 2019-01-03 |
| 卷号 | 243页码:320-326 |
| ISSN号 | 0304-4238 |
| 关键词 | Abiotic stress Amur grape Antioxidant enzyme Cold stress Low temperature |
| DOI | 10.1016/j.scienta.2018.08.055 |
| 英文摘要 | Low temperature is a vital environmental factor that affects plant growth, development and crop yields. Ethylene response factor (ERF) is a plant specific transcription factor with important roles in stress adaptation. However, the regulatory mechanism on how grapevine responds to cold stress is still unclear. In this study, we isolated and characterized two ERF genes from Vitis amurensis designated as VaERF080 and VaERF087. The function of these two genes was investigated in transgenic Arabidopsis. We found that both VaERF080 and VaERF087 were induced by cold stress and ethylene. The overexpression of these genes in Arabidopsis resulted in enhanced cold tolerance. The VaERF080- and VaERF087-overexpressing (OE) Arabidopsis lines showed lower levels of malondialdehyde (MDA) content and higher activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) under cold stress compared to wild-type (WT) Arabidopsis. Nine representative cold-responsive genes, CBF1, CBF2, ICE1, ZAT12, KIN1, SIZ1, RD29A, COR15A, and COR47, were significantly upregulated in OE Arabidopsis. These results revealed that both VaERF080 and VaERF087 may improve the cold tolerance of transgenic Arabidopsis through increasing antioxidant enzyme activities and regulating the expression of cold-related genes. |
| 资助项目 | Youth Innovation Promotion Association of CAS[2015281] ; China Postdoctoral Science Foundation[2016M601166] ; Grape Breeding Project of Ningxia[NXNYYZ201502] ; Open Project Program of the Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology (Fujian Agriculture and Forestry University) |
| WOS研究方向 | Agriculture |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000450381800039 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/3454]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Xin, Haiping |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 2.Fujian Agr & Forestry Univ, Ctr Genom & Biotechnol, Haixia Inst Sci & Technol, Fujian Prov Key Lab Haixia Appl Plant Syst Biol, Fuzhou 350002, Fujian, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Xiaoming,Zhu, Zhenfei,Zhang, Langlang,et al. Overexpression of ethylene response factors VaERF080 and VaERF087 from Vitis amurensis enhances cold tolerance in Arabidopsis[J]. SCIENTIA HORTICULTURAE,2019,243:320-326. |
| APA | Sun, Xiaoming.,Zhu, Zhenfei.,Zhang, Langlang.,Fang, Linchuan.,Zhang, Jisen.,...&Xin, Haiping.(2019).Overexpression of ethylene response factors VaERF080 and VaERF087 from Vitis amurensis enhances cold tolerance in Arabidopsis.SCIENTIA HORTICULTURAE,243,320-326. |
| MLA | Sun, Xiaoming,et al."Overexpression of ethylene response factors VaERF080 and VaERF087 from Vitis amurensis enhances cold tolerance in Arabidopsis".SCIENTIA HORTICULTURAE 243(2019):320-326. |
入库方式： OAI收割
来源：武汉植物园
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