Deletion of Pr72 causes cardiac developmental defects in Zebrafish
文献类型：期刊论文
| 作者 | Song, Guibo1; Han, Mingjun1; Li, Zuhua1; Gan, Xuedong4; Chen, Xiaowen3; Yang, Jie1; Dong, Sufang1; Yan, Ming1; Wan, Jun2; Wang, Yanggan4 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2018-11-27 |
| 卷号 | 13期号:11页码:12 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0206883 |
| 英文摘要 | The alpha regulator subunit B'' of protein phosphatase 2 (PPP2R3A), a regulatory subunit of protein phosphatase 2A (PP2A), was reported to present a special subcellular localization in cardiomyocytes and elevate in non-ischemia failing hearts. PPP2R3A has two transcriptions PR72 and PR130. PR72 acts as a negative regulator of the Wnt signaling cascade, while the Wnt signaling cascade plays a pivotal role in cardiac development. And PR130 was found to be involved in cardiac development of zebrafish in our previous study. Thus, to investigate the function of PR72 in heart, two stable pr72 knockout (KO) zebrafish lines were generated using Transcription Activator-Like Effector Nuclease (TALEN) technology. Homozygous pr72 KO fish struggled to survive to adulthood and exhibited cardiac developmental defects, including enlarged ventricular chambers, reduced cardiomyocytes and decreased cardiac function. And the defective sarcomere ultrastructure that affected mitochondria, I bands, Z lines, and intercalated disks was also observed. Furthermore, the abnormal heart looping was detected in mutants which could be rescued by injection with wild type pr72 mRNA. Additionally, it was found that Wnt effectors were elevated in mutants. Those indicated that deletion of pr72 in zebrafish interrupted cardiac development, probably through activation of the Wnt pathway. |
| 资助项目 | National Natural Science Foundation of China[81472024] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000451440000007 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/3473]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Yin, Zhan; Zheng, Fang |
| 作者单位 | 1.Wuhan Univ, Ctr Gene Diag, Zhongnan Hosp, Wuhan, Hubei, Peoples R China 2.Wuhan Univ, Dept Cardiol, Renmin Hosp, Wuhan, Hubei, Peoples R China 3.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Hubei, Peoples R China 4.Wuhan Univ, Dept Cardiol, Zhongnan Hosp, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Guibo,Han, Mingjun,Li, Zuhua,et al. Deletion of Pr72 causes cardiac developmental defects in Zebrafish[J]. PLOS ONE,2018,13(11):12. |
| APA | Song, Guibo.,Han, Mingjun.,Li, Zuhua.,Gan, Xuedong.,Chen, Xiaowen.,...&Zheng, Fang.(2018).Deletion of Pr72 causes cardiac developmental defects in Zebrafish.PLOS ONE,13(11),12. |
| MLA | Song, Guibo,et al."Deletion of Pr72 causes cardiac developmental defects in Zebrafish".PLOS ONE 13.11(2018):12. |
入库方式： OAI收割
来源：武汉植物园
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