Guidelines for the use and interpretation of assays for monitoring autophagy
文献类型:期刊论文
作者 | Klionsky, Daniel J.858; Abdalla, Fabio C.861; Abeliovich, Hagai862; Abraham, Robert T.863; Acevedo-Arozena, Abraham864; Adeli, Khosrow865; Agholme, Lotta866; Agnello, Maria867; Agostinis, Patrizia868; Aguirre-Ghiso, Julio A.869,870 |
刊名 | AUTOPHAGY |
出版日期 | 2012-04-01 |
卷号 | 8期号:4页码:445-544 |
ISSN号 | 1554-8627 |
关键词 | autolysosome autophagosome flux LC3 lysosome phagophore stress vacuole |
DOI | 10.4161/auto.19496 |
英文摘要 | In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field. |
资助项目 | National Institutes of Health Public Health Service[GM53396] |
WOS研究方向 | Cell Biology |
语种 | 英语 |
出版者 | LANDES BIOSCIENCE |
WOS记录号 | WOS:000305403400002 |
源URL | [http://202.127.146.157/handle/2RYDP1HH/3866] |
专题 | 中国科学院武汉植物园 |
通讯作者 | Klionsky, Daniel J. |
作者单位 | 1.Dulbecco Telethon Inst, Padua, Italy 2.Univ Milan, Dept Biomol Sci & Biotechnol, Milan, Italy 3.Buck Inst Res Aging, Novato, CA USA 4.Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden 5.Harvard Univ, Sch Med, Dept Genet, Boston, MA USA 6.Univ Texas MD Anderson Canc Ctr, Dept Melanoma Med Oncol, Houston, TX 77030 USA 7.Penn State Coll Med, Penn State Hershey Med Ctr, Dept Med Hematol Oncol, Lab Translat Oncol & Expt Canc Therapeut, Hershey, PA USA 8.Weizmann Inst Sci, Dept Biol Chem, IL-76100 Rehovot, Israel 9.Univ Milan, European Inst Oncol, Milan, Italy 10.Univ Stellenbosch, Dept Physiol Sci, ZA-7600 Stellenbosch, South Africa |
推荐引用方式 GB/T 7714 | Klionsky, Daniel J.,Abdalla, Fabio C.,Abeliovich, Hagai,et al. Guidelines for the use and interpretation of assays for monitoring autophagy[J]. AUTOPHAGY,2012,8(4):445-544. |
APA | Klionsky, Daniel J..,Abdalla, Fabio C..,Abeliovich, Hagai.,Abraham, Robert T..,Acevedo-Arozena, Abraham.,...&Sheng, Zu-Hang.(2012).Guidelines for the use and interpretation of assays for monitoring autophagy.AUTOPHAGY,8(4),445-544. |
MLA | Klionsky, Daniel J.,et al."Guidelines for the use and interpretation of assays for monitoring autophagy".AUTOPHAGY 8.4(2012):445-544. |
入库方式: OAI收割
来源:武汉植物园
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