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Chinese Academy of Sciences Institutional Repositories Grid
Guidelines for the use and interpretation of assays for monitoring autophagy

文献类型:期刊论文

作者Klionsky, Daniel J.858; Abdalla, Fabio C.861; Abeliovich, Hagai862; Abraham, Robert T.863; Acevedo-Arozena, Abraham864; Adeli, Khosrow865; Agholme, Lotta866; Agnello, Maria867; Agostinis, Patrizia868; Aguirre-Ghiso, Julio A.869,870
刊名AUTOPHAGY
出版日期2012-04-01
卷号8期号:4页码:445-544
ISSN号1554-8627
关键词autolysosome autophagosome flux LC3 lysosome phagophore stress vacuole
DOI10.4161/auto.19496
英文摘要In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
资助项目National Institutes of Health Public Health Service[GM53396]
WOS研究方向Cell Biology
语种英语
出版者LANDES BIOSCIENCE
WOS记录号WOS:000305403400002
源URL[http://202.127.146.157/handle/2RYDP1HH/3866]  
专题中国科学院武汉植物园
通讯作者Klionsky, Daniel J.
作者单位1.Dulbecco Telethon Inst, Padua, Italy
2.Univ Milan, Dept Biomol Sci & Biotechnol, Milan, Italy
3.Buck Inst Res Aging, Novato, CA USA
4.Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden
5.Harvard Univ, Sch Med, Dept Genet, Boston, MA USA
6.Univ Texas MD Anderson Canc Ctr, Dept Melanoma Med Oncol, Houston, TX 77030 USA
7.Penn State Coll Med, Penn State Hershey Med Ctr, Dept Med Hematol Oncol, Lab Translat Oncol & Expt Canc Therapeut, Hershey, PA USA
8.Weizmann Inst Sci, Dept Biol Chem, IL-76100 Rehovot, Israel
9.Univ Milan, European Inst Oncol, Milan, Italy
10.Univ Stellenbosch, Dept Physiol Sci, ZA-7600 Stellenbosch, South Africa
推荐引用方式
GB/T 7714
Klionsky, Daniel J.,Abdalla, Fabio C.,Abeliovich, Hagai,et al. Guidelines for the use and interpretation of assays for monitoring autophagy[J]. AUTOPHAGY,2012,8(4):445-544.
APA Klionsky, Daniel J..,Abdalla, Fabio C..,Abeliovich, Hagai.,Abraham, Robert T..,Acevedo-Arozena, Abraham.,...&Sheng, Zu-Hang.(2012).Guidelines for the use and interpretation of assays for monitoring autophagy.AUTOPHAGY,8(4),445-544.
MLA Klionsky, Daniel J.,et al."Guidelines for the use and interpretation of assays for monitoring autophagy".AUTOPHAGY 8.4(2012):445-544.

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