Interferon-Inducible Cholesterol-25-Hydroxylase Inhibits Hepatitis C Virus Replication via Distinct Mechanisms
文献类型：期刊论文
| 作者 | Chen, Yongzhi1,2; Wang, Shanshan; Yi, Zhaohong1; Tian, Huabin1,2; Aliyari, Roghiyh3; Li, Yanhua7; Chen, Gang7; Liu, Ping1; Zhong, Jin8; Chen, Xinwen9 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2014-12-03 |
| 卷号 | 4页码:8 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/srep07242 |
| 英文摘要 | Cholesterol 25-hydroxylase (CH25H) as an interferon-stimulated gene (ISG) has recently been shown to exert broad antiviral activity through the production of 25-hydroxycholesterol (25HC), which is believed to inhibit the virus-cell membrane fusion during viral entry. However, little is known about the function of CH25H on HCV infection and replication and whether antiviral function of CH25H is exclusively mediated by 25HC. In the present study, we have found that although 25HC produced by CH25H can inhibit HCV replication, CH25H mutants lacking the hydroxylase activity still carry the antiviral activity against HCV but not other viruses such as MHV-68. Further studies have revealed that CH25H can interact with the NS5A protein of HCV and inhibit its dimer formation, which is essential for HCV replication. Thus, our work has uncovered a novel mechanism by which CH25H restricts HCV replication, suggesting that CH25H inhibits viral infection through both 25HC-dependent and independent events. |
| 资助项目 | National Natural Science Foundation of China[81325012] ; Ministry of Science and Technology of China[2015CB554302] ; Ministry of Science and Technology of China[2009CB522505] ; Ministry of Science and Technology of China[2013CB911103] ; National Institutes of Health[AI078389] ; National Institutes of Health[AI069120] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000346264000001 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/3904]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Qin, F. Xiao-Feng |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Key Lab Infect & Immun, Beijing 100080, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA 4.Univ N Carolina, Dept Microbiol & Immunol, Chapel Hill, NC 27599 USA 5.Univ N Carolina, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA 6.Univ Texas MD Anderson Canc Ctr, Dept Immunol, Houston, TX 77030 USA 7.CAAS, Inst Qual Standard & Testing Technol Agroproduct, Beijing, Peoples R China 8.Chinese Acad Sci, Inst Pasteur Shanghai, Key Lab Mol Virol & Immunol, Shanghai, Peoples R China 9.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Yongzhi,Wang, Shanshan,Yi, Zhaohong,et al. Interferon-Inducible Cholesterol-25-Hydroxylase Inhibits Hepatitis C Virus Replication via Distinct Mechanisms[J]. SCIENTIFIC REPORTS,2014,4:8. |
| APA | Chen, Yongzhi.,Wang, Shanshan.,Yi, Zhaohong.,Tian, Huabin.,Aliyari, Roghiyh.,...&Cheng, Genhong.(2014).Interferon-Inducible Cholesterol-25-Hydroxylase Inhibits Hepatitis C Virus Replication via Distinct Mechanisms.SCIENTIFIC REPORTS,4,8. |
| MLA | Chen, Yongzhi,et al."Interferon-Inducible Cholesterol-25-Hydroxylase Inhibits Hepatitis C Virus Replication via Distinct Mechanisms".SCIENTIFIC REPORTS 4(2014):8. |
入库方式： OAI收割
来源：武汉植物园
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