Tung Tree (Vernicia fordii, Hemsl.) Genome and Transcriptome Sequencing Reveals Co-Ordinate Up-Regulation of Fatty Acid beta-Oxidation and Triacylglycerol Biosynthesis Pathways During Eleostearic Acid Accumulation in Seeds
文献类型：期刊论文
| 作者 | Cui, Peng1; Lin, Qiang2; Fang, Dongming1; Zhang, Lingling3; Li, Rongjun3; Cheng, Junyong4; Gao, Fei1; Shockey, Jay5; Hu, Songnian2,6; Lu, Shiyou3,7 |
| 刊名 | PLANT AND CELL PHYSIOLOGY
 |
| 出版日期 | 2018-10-01 |
| 卷号 | 59期号:10页码:1990-2003 |
| ISSN号 | 0032-0781 |
| 关键词 | Beta-oxidation Eleostearate Fatty acid Triacylglycerol Tung tree |
| DOI | 10.1093/pcp/pcy117 |
| 英文摘要 | The tung tree (Vernicia fordii) is one of only a few plant species that produces high oil-yielding seeds rich in a-eleostearic acid (alpha-ESA, 18:3 Delta(9cis,) (11trans, 13trans)), a conjugated trienoic fatty acid with valuable industrial and medical properties. Previous attempts have been made to engineer tung oil biosynthesis in transgenic oilseed crops, but these efforts have met with limited success. Here we present a high-quality genome assembly and developing seed transcriptomic data set for this species. Whole-genome shotgun sequencing generated 176 Gb of genome sequence data used to create a final assembled sequence 1,176,320 kb in size, with a scaffold N50 size of > 474 kb, and containing approximately 47,000 protein-coding genes. Genomic and transcriptomic data revealed full-length candidate genes for most of the known and suspected reactions that are necessary for fatty acid desaturation/conjugation, acyl editing and triacylglycerol biosynthesis. Seed transcriptomic analyses also revealed features unique to tung tree, including unusual transcriptional profiles of fatty acid biosynthetic genes, and coordinated (and seemingly paradoxical) simultaneous up-regulation of both fatty acid beta-oxidation and triacylglycerol biosynthesis in mid-development seeds. The precise temporal control of the expression patterns for these two pathways may account for alpha-ESA enrichment in tung seeds, while controlling the levels of potentially toxic by-products. Deeper understanding of these processes may open doors to the design of engineered oilseeds containing high levels of alpha-ESA. |
| 资助项目 | National Key R&D Program of China[2017YFD0600703] ; Chinese Academy of Sciences ['the Hundred Talents Program'] ; Chinese Academy of Agricultural Sciences [the Elite Youth Program] |
| WOS研究方向 | Plant Sciences ; Cell Biology |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS |
| WOS记录号 | WOS:000448668100007 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/3996]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Hu, Songnian; Lu, Shiyou |
| 作者单位 | 1.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Guangdong, Peoples R China 2.Chinese Acad Sci, Beijing Inst Genom, CAS Key Lab Genome Sci & Informat, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China 4.Hubei Acad Forestry, Wuhan 430075, Hubei, Peoples R China 5.ARS, USDA, Southern Reg Res Ctr, New Orleans, LA USA 6.Univ Chinese Acad Sci, Beijing 101407, Peoples R China 7.Chinese Acad Sci, Sinoafrica Joint Res Ctr, Wuhan 430074, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Peng,Lin, Qiang,Fang, Dongming,et al. Tung Tree (Vernicia fordii, Hemsl.) Genome and Transcriptome Sequencing Reveals Co-Ordinate Up-Regulation of Fatty Acid beta-Oxidation and Triacylglycerol Biosynthesis Pathways During Eleostearic Acid Accumulation in Seeds[J]. PLANT AND CELL PHYSIOLOGY,2018,59(10):1990-2003. |
| APA | Cui, Peng.,Lin, Qiang.,Fang, Dongming.,Zhang, Lingling.,Li, Rongjun.,...&Lu, Shiyou.(2018).Tung Tree (Vernicia fordii, Hemsl.) Genome and Transcriptome Sequencing Reveals Co-Ordinate Up-Regulation of Fatty Acid beta-Oxidation and Triacylglycerol Biosynthesis Pathways During Eleostearic Acid Accumulation in Seeds.PLANT AND CELL PHYSIOLOGY,59(10),1990-2003. |
| MLA | Cui, Peng,et al."Tung Tree (Vernicia fordii, Hemsl.) Genome and Transcriptome Sequencing Reveals Co-Ordinate Up-Regulation of Fatty Acid beta-Oxidation and Triacylglycerol Biosynthesis Pathways During Eleostearic Acid Accumulation in Seeds".PLANT AND CELL PHYSIOLOGY 59.10(2018):1990-2003. |
入库方式： OAI收割
来源：武汉植物园
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