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Identification of the caveolae/raft-mediated endocytosis as the primary entry pathway for aquareovirus

文献类型：期刊论文

作者Zhang, Jie1,2; Fang, Qin1; Zhang, Fuxian1; Guo, Hong1; Chen, Qingxiu1
刊名VIROLOGY
出版日期2018
卷号513页码:195-207
关键词DsRNA virus Reovirus Aquareovirus Cell entry Quantum dot Caveolae/raft-mediated endocytosis
ISSN号0042-6822
DOI10.1016/j.virol.2017.09.019
英文摘要Grass carp reovirus (GCRV), a member of the Aquareovirus genus in the Reoviridae family, is considered the most pathogenic aquareovirus. However, its productive viral entry pathways remain largely unclear. Using a combination of quantum dot (QD)-based live-virus tracking and biochemical assays, we found that extraction of cellular membrane cholesterol with methyl-beta-cyclodextrin (M beta CD) and nystatin strongly inhibited the internalization of GCRVs, and supplementation with cholesterol restored viral infection. In addition, the entry of the virus was restrained by genistein, an inhibitor known to block caveolar endocytosis. Subsequent real-time tracking experiments revealed that the QD-labeled GCRV particles were colocalized with caveolin-1, and transfection of cells with dominant-negative mutant (caveolin-1 Y14F) significantly reduced GCRV infection. In contrast, no effects on virus infection were detected when the clathrin-mediated endocytosis or the macro-pinocytosis inhibitors were used. Our results collectively suggest that aquareoviruses can use caveolae/raft-mediated endocytosis as the primary entry pathway to initiate productive infection.
资助项目National Natural Science Foundation of China[31372565] ; National Natural Science Foundation of China[31400139] ; National Natural Science Foundation of China[31370190] ; National Natural Science Foundation of China[31672693] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2013FB05]
WOS研究方向Virology
语种英语
WOS记录号WOS:000417670400022
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
源URL[http://202.127.146.157/handle/2RYDP1HH/4418]  
专题中国科学院武汉植物园
通讯作者Fang, Qin
作者单位1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Jie,Fang, Qin,Zhang, Fuxian,et al. Identification of the caveolae/raft-mediated endocytosis as the primary entry pathway for aquareovirus[J]. VIROLOGY,2018,513:195-207.
APA Zhang, Jie,Fang, Qin,Zhang, Fuxian,Guo, Hong,&Chen, Qingxiu.(2018).Identification of the caveolae/raft-mediated endocytosis as the primary entry pathway for aquareovirus.VIROLOGY,513,195-207.
MLA Zhang, Jie,et al."Identification of the caveolae/raft-mediated endocytosis as the primary entry pathway for aquareovirus".VIROLOGY 513(2018):195-207.

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来源：武汉植物园

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