Discovery of a rich gene pool of bat SARS-related coronaviruses provides new insights into the origin of SARS coronavirus
文献类型：期刊论文
| 作者 | Hu, Ben1; Zeng, Lei-Ping1; Yang, Xing-Lou1; Ge, Xing-Yi1; Zhang, Wei1; Li, Bei1; Xie, Jia-Zheng1; Shen, Xu-Rui1; Zhang, Yun-Zhi2,3; Wang, Ning1 |
| 刊名 | PLOS PATHOGENS
 |
| 出版日期 | 2017-11-01 |
| 卷号 | 13期号:11页码:27 |
| ISSN号 | 1553-7366 |
| DOI | 10.1371/journal.ppat.1006698 |
| 英文摘要 | A large number of SARS-related coronaviruses (SARSr-CoV) have been detected in horse-shoe bats since 2005 in different areas of China. However, these bat SARSr-CoVs show sequence differences from SARS coronavirus (SARS-CoV) in different genes (S, ORF8, ORF3, etc) and are considered unlikely to represent the direct progenitor of SARS-CoV. Herein, we report the findings of our 5-year surveillance of SARSr-CoVs in a cave inhabited by multiple species of horseshoe bats in Yunnan Province, China. The full-length genomes of 11 newly discovered SARSr-CoV strains, together with our previous findings, reveals that the SARSr-CoVs circulating in this single location are highly diverse in the S gene, ORF3 and ORF8. Importantly, strains with high genetic similarity to SARS-CoV in the hypervariable N-terminal domain (NTD) and receptor-binding domain (RBD) of the S1 gene, the ORF3 and ORF8 region, respectively, were all discovered in this cave. In addition, we report the first discovery of bat SARSr-CoVs highly similar to human SARS-CoV in ORF3b and in the split ORF8a and 8b. Moreover, SARSr-CoV strains from this cave were more closely related to SARS-CoV in the non-structural protein genes ORF1a and 1b compared with those detected elsewhere. Recombination analysis shows evidence of frequent recombination events within the S gene and around the ORF8 between these SARSr-CoVs. We hypothesize that the direct progenitor of SARS-CoV may have originated after sequential recombination events between the precursors of these SARSr-CoVs. Cell entry studies demonstrated that three newly identified SARSr-CoVs with different S protein sequences are all able to use human ACE2 as the receptor, further exhibiting the close relationship between strains in this cave and SARS-CoV. This work provides new insights into the origin and evolution of SARS-CoV and highlights the necessity of preparedness for future emergence of SARS-like diseases. |
| 资助项目 | National Natural Science Foundation of China[81290341] ; National Natural Science Foundation of China[31621061] ; China Mega-Project for Infectious Disease[2014ZX10004001-003] ; Ministry of Science and Technology of China[2013FY113500] ; Chinese Academy of Sciences[XDPB0301] ; National Institutes of Health[NIAID R01AI110964] ; USAID Emerging Pandemic Threats (EPT) PREDICT ; CAS ; NRF-CRP[NRF-CRP10-2012-05] ; WIV One-Three-Five Strategic Program[WIV-135-TP1] |
| WOS研究方向 | Microbiology ; Parasitology ; Virology |
| 语种 | 英语 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| WOS记录号 | WOS:000416888500016 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/4442]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Cui, Jie; Shi, Zheng-Li |
| 作者单位 | 1.Chinese Acad Sci, Ctr Emerging Infect Dis, CAS Key Lab Special Pathogens & Biosafety, Wuhan Inst Virol, Wuhan, Peoples R China 2.Yunnan Inst Endem Dis Control & Prevent, Dali, Peoples R China 3.Dali Univ, Dali, Peoples R China 4.EcoHlth Alliance, New York, NY USA 5.Duke NUS Med Sch, Programme Emerging Infect Dis, Singapore, Singapore |
| 推荐引用方式 GB/T 7714 | Hu, Ben,Zeng, Lei-Ping,Yang, Xing-Lou,et al. Discovery of a rich gene pool of bat SARS-related coronaviruses provides new insights into the origin of SARS coronavirus[J]. PLOS PATHOGENS,2017,13(11):27. |
| APA | Hu, Ben.,Zeng, Lei-Ping.,Yang, Xing-Lou.,Ge, Xing-Yi.,Zhang, Wei.,...&Shi, Zheng-Li.(2017).Discovery of a rich gene pool of bat SARS-related coronaviruses provides new insights into the origin of SARS coronavirus.PLOS PATHOGENS,13(11),27. |
| MLA | Hu, Ben,et al."Discovery of a rich gene pool of bat SARS-related coronaviruses provides new insights into the origin of SARS coronavirus".PLOS PATHOGENS 13.11(2017):27. |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。