Live Visualization of HIV-1 Proviral DNA Using a Dual-Color-Labeled CRISPR System
文献类型：期刊论文
| 作者 | Li, Wei2; Zhang, Zhiping2; Zhang, Xiaowei2; Wu, Guoqiang2; Cui, Zongqiang2; Tan, Tianwei3; Zhang, Xian-En1; Ma, Yingxin2,3; Wang, Mingxiu2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2017-12-05 |
| 卷号 | 89期号:23页码:12896-12901 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.7b03584 |
| 英文摘要 | HIV latency is one of the major problems in HIV/AIDS cure. Imaging single-copy integrated proviral HIV DNA in host cell has both virology and clinical significance but remains technical challenge. Here, we developed a dual-color labeled CRISPR system to image the HIV-1 integrated proviral DNA in latently infected cells. The pair of CRISPRs was fluorescently labeled with two different color QDs using two alternative bioorthogonal ligation reactions. Integrated HIV-sequences are successfully mapped based on the colocalized signals of QDs in living cells. Compared to the existing zinc finger proteins and TALENs, the CRISPR system is much easier to operate and more efficient in imaging of internal genomic loci. Therefore, the proposed method proviral HIV-1, but also a versatile platform to image single genomic loci in living could be not cells. only a powerful tool for imaging |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000417549600041 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/4452]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Cui, Zongqiang; Zhang, Xian-En |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Ctr Biol Macromol, Natl Key Lab Biomacromol, Beijing, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China 3.Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Wei,Zhang, Zhiping,Zhang, Xiaowei,et al. Live Visualization of HIV-1 Proviral DNA Using a Dual-Color-Labeled CRISPR System[J]. ANALYTICAL CHEMISTRY,2017,89(23):12896-12901. |
| APA | Li, Wei.,Zhang, Zhiping.,Zhang, Xiaowei.,Wu, Guoqiang.,Cui, Zongqiang.,...&Wang, Mingxiu.(2017).Live Visualization of HIV-1 Proviral DNA Using a Dual-Color-Labeled CRISPR System.ANALYTICAL CHEMISTRY,89(23),12896-12901. |
| MLA | Li, Wei,et al."Live Visualization of HIV-1 Proviral DNA Using a Dual-Color-Labeled CRISPR System".ANALYTICAL CHEMISTRY 89.23(2017):12896-12901. |
入库方式： OAI收割
来源：武汉植物园
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