The uncoupling of catalysis and translocation in the viral RNA-dependent RNA polymerase
文献类型：期刊论文
| 作者 | Gong, Peng1; Shu, Bo1,2 |
| 刊名 | RNA BIOLOGY
 |
| 出版日期 | 2017 |
| 卷号 | 14期号:10页码:1314-1319 |
| 关键词 | Active site closure crystal structure nucleotide addition cycle RNA-dependent RNA polymerase translocation |
| ISSN号 | 1547-6286 |
| DOI | 10.1080/15476286.2017.1300221 |
| 英文摘要 | The nucleotide addition cycle of nucleic acid polymerases includes 2 major events: the pre-chemistry active site closure leading to the addition of one nucleotide to the product chain; the post-chemistry translocation step moving the polymerase active site one position downstream on its template. In viral RNA-dependent RNA polymerases (RdRPs), structural and biochemical evidences suggest that these 2 events are not tightly coupled, unlike the situation observed in A-family polymerases such as the bacteriophage T7 RNA polymerase. Recently, an RdRP translocation intermediate crystal structure of enterovirus 71 shed light on how translocation may be controlled by elements within RdRP catalytic motifs, and a series of poliovirus apo RdRP crystal structures explicitly suggest that a motif B loop may assist the movement of the template strand in late stages of transcription. Implications of RdRP catalysis-translocation uncoupling and the remaining challenges to further elucidate RdRP translocation mechanism are also discussed. |
| 资助项目 | National Key Basic Research Program of China[2013CB911100] ; National Key Research and Development Program of China[2016YFC1200400] ; One-Three-Five Strategic Program, Wuhan Institute of Virology, Chinese Academy of Sciences[Y605191SA1] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000418050000006 |
| 出版者 | TAYLOR & FRANCIS INC |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/4461]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Gong, Peng |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gong, Peng,Shu, Bo. The uncoupling of catalysis and translocation in the viral RNA-dependent RNA polymerase[J]. RNA BIOLOGY,2017,14(10):1314-1319. |
| APA | Gong, Peng,&Shu, Bo.(2017).The uncoupling of catalysis and translocation in the viral RNA-dependent RNA polymerase.RNA BIOLOGY,14(10),1314-1319. |
| MLA | Gong, Peng,et al."The uncoupling of catalysis and translocation in the viral RNA-dependent RNA polymerase".RNA BIOLOGY 14.10(2017):1314-1319. |
入库方式： OAI收割
来源：武汉植物园
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